論文 - 詳細
| RRC ID | 12432 |
|---|---|
| 著者 | Kamio N, Akifusa S, Yamaguchi N, Yamashita Y. |
| タイトル | Induction of granulocyte colony-stimulating factor by globular adiponectin via the MEK-ERK pathway. |
| ジャーナル | Mol Cell Endocrinol |
| Abstract |
Adiponectin, an adipocyte-derived cytokine, affects a number of physiological processes, including immune function and inflammation. We investigated whether globular adiponectin (gAd) affects the expression of inflammation-related genes in murine macrophages (RAW264 cells). DNA microarray analysis indicated that granulocyte colony-stimulating factor (G-CSF) showed the largest increase in expression in gAd-stimulated RAW264 cells. The gAd-induced secretion of G-CSF increased in a time- and dose-dependent manner. U0126 (MEK1/2 inhibitor) and PD98059 (MEK1 inhibitor) reduced the gAd-induced G-CSF mRNA expression and G-CSF protein production. gAd induced the phosphorylation of MEK1/2 and ERK1/2 in RAW264 cells. In addition, the gAd-induced phosphorylation of MEK1/2 and ERK1/2 was dramatically reduced by PD98059 and U0126, respectively. Collectively, these results suggest that MEK1/2-ERK1/2 signaling is involved in the adiponectin-induced secretion of G-CSF. |
| 巻・号 | 292(1-2) |
| ページ | 20-5 |
| 公開日 | 2008-9-24 |
| DOI | 10.1016/j.mce.2008.05.002 |
| PII | S0303-7207(08)00207-4 |
| PMID | 18555587 |
| MeSH | Adiponectin / pharmacology* Animals Cell Line Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Extracellular Signal-Regulated MAP Kinases / metabolism* Gene Expression Regulation / drug effects Granulocyte Colony-Stimulating Factor / genetics* Granulocyte Colony-Stimulating Factor / metabolism I-kappa B Kinase / metabolism Inflammation / genetics Intracellular Space / drug effects Intracellular Space / metabolism MAP Kinase Signaling System / drug effects Macrophages / drug effects Macrophages / enzymology Mice Mitogen-Activated Protein Kinase Kinases / metabolism* NF-kappa B / metabolism Phosphorylation / drug effects Time Factors |
| IF | 3.871 |
| 引用数 | 12 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |