論文 - 詳細
| RRC ID | 12439 |
|---|---|
| 著者 | Kina Y, Fukumoto S, Miura K, Tademoto S, Nunomura K, Dirgahayu P, Hirai K. |
| タイトル | A glycoprotein from Spirometra erinaceieuropaei plerocercoids suppresses osteoclastogenesis and proinflammatory cytokine gene expression. |
| ジャーナル | Int J Parasitol |
| Abstract |
Various parasites modify the immune-reactions of the host. We have previously shown that crude excretory/secretory (ES) products from plerocercoids of Spirometra erinaceieuropaei, the plerocercoids of which cause sparganosis in humans, suppress the expression of tumor necrosis factor (TNF)-alpha and IL-1beta in lipopolysaccharide (LPS)-stimulated macrophages. As osteoclasts are cells of the monocyte/macrophage lineage, we hypothesised that ES products might suppress receptor activator of nuclear factor kappaB ligand-induced osteoclastogenesis. Crude ES products from plerocercoids suppressed osteoclastogenesis, judged by tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cell counting, and the mature osteoclast-specific gene expression (calcitonin receptor and TRAP). Second, we purified the inhibitory factor for osteoclastogenesis from the crude ES products. The factor was a trypsin-sensitive glycoprotein and had a relative molecular mass of 90 kDa. The glycoprotein, plerocercoid-immunosuppressive factor, from crude ES products could suppress the gene expression of TNF-alpha, IL-1beta and NO synthesis in LPS-stimulated RAW264.7 macrophages. |
| 巻・号 | 35(13) |
| ページ | 1399-406 |
| 公開日 | 2005-11-1 |
| DOI | 10.1016/j.ijpara.2005.05.012 |
| PII | S0020-7519(05)00186-4 |
| PMID | 16051245 |
| MeSH | Animals Biological Factors / pharmacology Bone Marrow Cells / cytology Bone Marrow Cells / drug effects Carrier Proteins / antagonists & inhibitors Carrier Proteins / physiology Cell Differentiation / drug effects Cell Line Cytokines / biosynthesis* Cytokines / genetics Gene Expression Regulation / drug effects Glycoproteins / isolation & purification Glycoproteins / pharmacology* Helminth Proteins / isolation & purification Helminth Proteins / pharmacology* Interleukin-1 / biosynthesis Interleukin-1 / genetics Lipopolysaccharides / immunology Membrane Glycoproteins / antagonists & inhibitors Membrane Glycoproteins / physiology Mice Osteoclasts / drug effects* Osteoclasts / physiology RANK Ligand Receptor Activator of Nuclear Factor-kappa B Reverse Transcriptase Polymerase Chain Reaction / methods Spirometra / chemistry* Tumor Necrosis Factor-alpha / biosynthesis Tumor Necrosis Factor-alpha / genetics |
| IF | 3.53 |
| 引用数 | 9 |
| WOS 分野 | PARASITOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |