RRC ID 12441
Author Yamaguchi N, Hamachi T, Kamio N, Akifusa S, Masuda K, Nakamura Y, Nonaka K, Maeda K, Hanazawa S, Yamashita Y.
Title Expression levels of adiponectin receptors and periodontitis.
Journal J. Periodont. Res.
Abstract BACKGROUND AND OBJECTIVE:We recently showed that adiponectin, an adipocyte-derived cytokine, may function as a negative regulator of the Toll-like receptor signaling pathway and of osteoclast formation in periodontal disease. In this study, we investigated whether the expression levels of adiponectin receptors (AdipoR1 and AdipoR2) are related to the presence of periodontitis.
MATERIAL AND METHODS:We initially examined, using RT-PCR, the expression of the AdipoR1 and AdipoR2 genes at the mRNA level in several oral tissues of C57BL mice. Next, we investigated (using real-time PCR assays) whether inflammatory cytokines, such as tumor necrosis factor-alpha, could affect the expression levels of these genes in human gingival fibroblasts. Lastly, we compared the expression levels of these receptor proteins in gingival tissues between two healthy subjects and five patients with severe periodontal disease using western blotting analysis.
RESULTS:The AdipoR1 and AdipoR2 receptors were ubiquitously expressed in the oral tissues of mice. We observed that treatment with tumor necrosis factor-alpha could significantly reduce the expression levels of both AdipoR1 and AdipoR2 genes in human gingival fibroblasts. Moreover, we found that the expression of both receptors was lower in periodontal tissues from patients with severe periodontitis than in patients with healthy periodontal tissues.
CONCLUSION:These observations suggest that adiponectin may not function efficiently in sites of periodontal disease because of a decrease in the number of its receptors, and this probable dysfunction may play a role in worsening periodontitis in patients.
Volume 45(2)
Pages 296-300
Published 2010-4
DOI 10.1111/j.1600-0765.2009.01222.x
PMID 20470261
MeSH Aged Animals Cell Line Cells, Cultured Chronic Periodontitis / metabolism Cytokines / pharmacology Female Fibroblasts / metabolism Gingiva / metabolism Gingivitis / metabolism Humans Inflammation Mediators / pharmacology Male Mice Mice, Inbred C57BL Middle Aged Periodontal Pocket / metabolism Periodontitis / metabolism* Receptors, Adiponectin / analysis* Receptors, Adiponectin / drug effects Tumor Necrosis Factor-alpha / pharmacology
IF 2.878
Times Cited 9
Human and Animal Cells RAW 264 (RCB0535)