RRC ID 12442
Author Kim YH, Choi EM.
Title Stimulation of osteoblastic differentiation and inhibition of interleukin-6 and nitric oxide in MC3T3-E1 cells by pomegranate ethanol extract.
Journal Phytother Res
Abstract In this experiment, we studied the effects of pomegranate fruit extract (PE) on the function of osteoblastic MC3T3-E1 cells and the production of local factors in osteoblasts. PE (16 approximately 250 microg/ml) significantly increased the growth of MC3T3-E1 cells (P < 0.05). Moreover, PE (50 microg/ml) caused a significant elevation of alkaline phosphatase (ALP) activity and collagen content in the cells. We then examined the effect of PE on the TNF-alpha-induced production of interleukin-6 (IL-6) and nitric oxide (NO) in osteoblasts. Treatment with PE (10 approximately 50 microg/ml) decreased the TNF-alpha (10(-10) M)-induced production of IL-6 and NO in osteoblasts.
Volume 23(5)
Pages 737-9
Published 2009-5
DOI 10.1002/ptr.2587
PMID 19107859
MeSH Alkaline Phosphatase / metabolism Animals Cell Differentiation / drug effects* Cell Line Collagen / metabolism Interleukin-6 / metabolism* Mice Nitric Oxide / metabolism* Osteoblasts / drug effects* Plant Extracts / pharmacology* Punicaceae / chemistry Tumor Necrosis Factor-alpha / metabolism
IF 3.349
Times Cited 6
Human and Animal Cells MC3T3-E1 (RCB1126)