論文 - 詳細
| RRC ID | 12447 |
|---|---|
| 著者 | Fernandes H, Dechering K, Van Someren E, Steeghs I, Apotheker M, Leusink A, Bank R, Janeczek K, Van Blitterswijk C, de Boer J. |
| タイトル | The role of collagen crosslinking in differentiation of human mesenchymal stem cells and MC3T3-E1 cells. |
| ジャーナル | Tissue Eng Part A |
| Abstract |
Collagen is the main protein component of the extracellular matrix of bone, and it has structural and instructive properties. Collagen undergoes many post-translational modifications, including extensive crosslinking. Although defective crosslinking has been implicated in human syndromes (e.g., osteogenesis imperfecta or Ehlers-Danlos syndrome), it is not clear to what extent crosslinking is necessary for collagen's instructive properties during bone formation. Here we report that inhibition of collagen crosslinking in the mouse pre-osteoblast cell line MC3T3-E1 impairs the osteogenic program. Genome-wide expression profiling of beta-aminopropionitrile-treated and control cells revealed that matrix deposition by MC3T3-E1 cells provides a feed back signal, driving cells through the differentiation process, that is strongly impaired when crosslinking is inhibited. Inhibition of crosslinking did not affect osteogenic differentiation of human mesenchymal stem cells (hMSCs), shown by the expression of alkaline phosphatase and genome-wide gene expression analysis, although it enhances matrix mineralization. In conclusion, collagen crosslinking harbors instructive properties in MC3T3-E1 differentiation but plays a more-passive role in differentiation of bone marrow-derived hMSCs. |
| 巻・号 | 15(12) |
| ページ | 3857-67 |
| 公開日 | 2009-12-1 |
| DOI | 10.1089/ten.tea.2009.0011 |
| PMID | 19694522 |
| MeSH | Animals Cell Differentiation* / drug effects Collagen / metabolism* Cross-Linking Reagents / metabolism* Enzyme Inhibitors / pharmacology Extracellular Matrix / drug effects Extracellular Matrix / genetics Gene Expression Profiling Gene Expression Regulation / drug effects Humans Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / drug effects Mesenchymal Stem Cells / enzymology Mesenchymal Stem Cells / metabolism* Mice Models, Biological Osteoblasts / cytology* Osteoblasts / drug effects Osteoblasts / enzymology Osteoblasts / metabolism* Osteogenesis / drug effects Protein-Lysine 6-Oxidase / antagonists & inhibitors |
| IF | 3.508 |
| 引用数 | 35 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY CELL & TISSUE ENGINEERING CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |