RRC ID 12461
Author Carpenter M, Rao MS, Freed W, Zeng X.
Title Derivation and characterization of neuronal precursors and dopaminergic neurons from human embryonic stem cells in vitro.
Journal Methods Mol. Biol.
Abstract Dopaminergic neurons of human origin have many potential research applications such as in vitro studies on biochemical pathways related to neuronal disorders, and potentially direct cell replacement for therapeutic use. Dopaminergic neurons with apparently normal properties can be produced from embryonic stem cells from mice and sub-human primates by the simple procedure of coculturing with the PA6 stromal cell line. Recently, we have demonstrated that this coculture system can induce dopaminergic differentiation in human embryonic stem cells, and the human embryonic stem cell-derived dopaminergic cells exhibit biochemical and functional properties of mature dopaminergic neurons.
Volume 331
Pages 153-67
Published 2006
DOI 10.1385/1-59745-046-4:153
PMID 16881516
MeSH Animals Cell Differentiation / physiology Cell Line Coculture Techniques / methods* Cryopreservation / methods Dopamine / physiology* Gene Expression Profiling Humans Mice Neurons / cytology* Neurons / physiology Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / physiology Stromal Cells / cytology*
Human and Animal Cells