| RRC ID |
12477
|
| 著者 |
Takegawa K, Tohda H, Sasaki M, Idiris A, Ohashi T, Mukaiyama H, Giga-Hama Y, Kumagai H.
|
| タイトル |
Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system.
|
| ジャーナル |
Biotechnol Appl Biochem
|
| Abstract |
The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc. pombe is considered an attractive host for the production of heterologous proteins. Expression vectors for high-level expression in Sc. pombe have been developed and many foreign proteins have been successfully expressed. However, further improvements in the protein-expressing host systems are still required for the production of heterologous proteins involved in post-translational modification, metabolism and intracellular trafficking. This minireview focuses on recent advances in heterologous protein production by use of engineered fission-yeast strains.
|
| 巻・号 |
53(Pt 4)
|
| ページ |
227-35
|
| 公開日 |
2009-6-22
|
| DOI |
10.1042/BA20090048
|
| PII |
BA20090048
|
| PMID |
19531030
|
| MeSH |
Genetic Engineering
Genetic Vectors / genetics
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Recombinant Proteins / biosynthesis*
Recombinant Proteins / genetics*
Schizosaccharomyces / genetics*
Schizosaccharomyces / metabolism
|
| IF |
1.638
|
| 引用数 |
42
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 酵母 |
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