RRC ID 12522
著者 Song JS, Liu X, Liu XS, He X.
タイトル A high-resolution map of nucleosome positioning on a fission yeast centromere.
ジャーナル Genome Res
Abstract A key element for defining the centromere identity is the incorporation of a specific histone H3, CENPA, known as Cnp1p in Schizosaccharomyces pombe. Previous studies have suggested that functional S. pombe centromeres lack regularly positioned nucleosomes and may involve chromatin remodeling as a key step of kinetochore assembly. We used tiling microarrays to show that nucleosomes are, in fact, positioned in regular intervals in the core of centromere 2, providing the first high-resolution map of regional centromere chromatin. Nucleosome locations are not disrupted by mutations in kinetochore protein genes cnp1, mis18, mis12, nuf2, mal2; overexpression of cnp1; or the deletion of ams2, which encodes a GATA-like factor participating in CENPA incorporation. Bioinformatics analysis of the centromere sequence indicates certain enriched motifs in linker regions between nucleosomes and reveals a sequence bias in nucleosome positioning. In addition, sequence analysis of nucleosome-free regions identifies novel binding sites of Ams2p. We conclude that centromeric nucleosome positions are stable and may be derived from the underlying DNA sequence.
巻・号 18(7)
ページ 1064-72
公開日 2008-7-1
DOI 10.1101/gr.075374.107
PII gr.075374.107
PMID 18411404
PMC PMC2493395
MeSH Centromere / genetics* Chromosome Mapping* Chromosomes, Fungal / genetics* DNA, Fungal / analysis DNA, Fungal / genetics Nucleosomes / genetics* Schizosaccharomyces / genetics*
IF 11.093
引用数 22
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 FY11157 FY8496 FY11258 FY11198