論文 - 詳細
| RRC ID | 12522 |
|---|---|
| 著者 | Song JS, Liu X, Liu XS, He X. |
| タイトル | A high-resolution map of nucleosome positioning on a fission yeast centromere. |
| ジャーナル | Genome Res |
| Abstract |
A key element for defining the centromere identity is the incorporation of a specific histone H3, CENPA, known as Cnp1p in Schizosaccharomyces pombe. Previous studies have suggested that functional S. pombe centromeres lack regularly positioned nucleosomes and may involve chromatin remodeling as a key step of kinetochore assembly. We used tiling microarrays to show that nucleosomes are, in fact, positioned in regular intervals in the core of centromere 2, providing the first high-resolution map of regional centromere chromatin. Nucleosome locations are not disrupted by mutations in kinetochore protein genes cnp1, mis18, mis12, nuf2, mal2; overexpression of cnp1; or the deletion of ams2, which encodes a GATA-like factor participating in CENPA incorporation. Bioinformatics analysis of the centromere sequence indicates certain enriched motifs in linker regions between nucleosomes and reveals a sequence bias in nucleosome positioning. In addition, sequence analysis of nucleosome-free regions identifies novel binding sites of Ams2p. We conclude that centromeric nucleosome positions are stable and may be derived from the underlying DNA sequence. |
| 巻・号 | 18(7) |
| ページ | 1064-72 |
| 公開日 | 2008-7-1 |
| DOI | 10.1101/gr.075374.107 |
| PII | gr.075374.107 |
| PMID | 18411404 |
| PMC | PMC2493395 |
| MeSH | Centromere / genetics* Chromosome Mapping* Chromosomes, Fungal / genetics* DNA, Fungal / analysis DNA, Fungal / genetics Nucleosomes / genetics* Schizosaccharomyces / genetics* |
| IF | 11.093 |
| 引用数 | 22 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY11157 FY8496 FY11258 FY11198 |