RRC ID 12532
Author Davis L, Rozalén AE, Moreno S, Smith GR, Martín-Castellanos C.
Title Rec25 and Rec27, novel linear-element components, link cohesin to meiotic DNA breakage and recombination.
Journal Curr. Biol.
Abstract Meiosis is a specialized nuclear division by which sexually reproducing diploid organisms generate haploid gametes. Recombination between homologous chromosomes facilitates accurate meiotic chromosome segregation and is initiated by DNA double-strand breaks (DSBs) made by the conserved topoisomerase-like protein Spo11 (Rec12 in fission yeast), but DSBs are not evenly distributed across the genome. In Schizosaccharomyces pombe, proteinaceous structures known as linear elements (LinEs) are formed during meiotic prophase. The meiosis-specific cohesin subunits Rec8 and Rec11 are essential for DSB formation in some regions of the genome, as well as for formation of LinEs or the related synaptonemal complex (SC) in other eukaryotes. Proteins required for DSB formation decorate LinEs, and mutants lacking Rec10, a major component of LinEs, are completely defective for recombination. Although recombination may occur in the context of LinEs, it is not well understood how Rec10 is loaded onto chromosomes. We describe two novel components of LinEs in fission yeast, Rec25 and Rec27. Comparisons of rec25Delta, rec27Delta, and rec10Delta mutants suggest multiple pathways to load Rec10. In the major pathway, Rec10 is loaded, together with Rec25 and Rec27, in a Rec8-dependent manner with subsequent region-specific effects on recombination.
Volume 18(11)
Pages 849-54
Published 2008-6-3
DOI 10.1016/j.cub.2008.05.025
PII S0960-9822(08)00663-5
PMID 18514516
PMC PMC3119532
MeSH Cell Cycle Proteins / metabolism* Chromosomal Proteins, Non-Histone / metabolism* Chromosome Pairing / physiology DNA Breaks, Double-Stranded Meiosis / physiology* Nuclear Proteins / metabolism* Phosphoproteins / metabolism Recombination, Genetic Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / metabolism*
IF 9.193
Times Cited 21
Yeast ?