RRC ID 12577
Author Alao JP, Huis In 't Veld PJ, Buhse F, Sunnerhagen P.
Title Hyperosmosis enhances radiation and hydroxyurea resistance of Schizosaccharomyces pombe checkpoint mutants through the spindle checkpoint and delayed cytokinesis.
Journal Mol Microbiol
Abstract The DNA damage and stress response pathways interact to regulate cellular responses to genotoxins and environmental stresses. How these pathways interact in Schizosaccharomyces pombe is not well understood. We demonstrate that osmotic stress suppresses the DNA damage sensitivity of checkpoint mutants, and that this occurs through three distinct cell cycle delays. A delay in G2/M is dependent on Srk1. Progression through mitosis is halted by the Mad2-dependent spindle checkpoint. Finally, cytokinesis is impaired by modulating Cdc25 expression. These three delays, imposed by osmotic stress, together compensate for the loss of checkpoint signalling.
Volume 77(1)
Pages 143-57
Published 2010-7-1
DOI 10.1111/j.1365-2958.2010.07193.x
PMID 20444100
MeSH Cell Cycle Proteins / metabolism* Cytokinesis / drug effects* Cytokinesis / radiation effects* Hydroxyurea / toxicity* Mad2 Proteins Mitogen-Activated Protein Kinases Nuclear Proteins Osmotic Pressure* Schizosaccharomyces / drug effects Schizosaccharomyces / growth & development Schizosaccharomyces / physiology* Schizosaccharomyces / radiation effects Schizosaccharomyces pombe Proteins / metabolism Ultraviolet Rays* cdc25 Phosphatases
IF 3.418
Times Cited 6
Yeast wee1D mad2D rad13D rad24D cdc13-HA cdc25-HA cut2-HA cut2-364 cdc2-3w cdc2-3w cdc25D nda3-KM311 nda3-KM311 rad3D