論文 - 詳細
| RRC ID | 12582 |
|---|---|
| 著者 | Suzuki S, Matsuzawa T, Nukigi Y, Takegawa K, Tanaka N. |
| タイトル | Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast. |
| ジャーナル | Microbiology (Reading) |
| Abstract |
Schizosaccharomyces species are currently the only known organisms with two types of genes encoding UDP-glucose/-galactose 4-epimerase, uge1(+) and gal10(+). A strain deleted for uge1(+) exhibited a severe galactosylation defect and a decrease in activity and in UDP-galactose content when grown in glucose-rich medium (2 % glucose), indicating that Uge1p is a major UDP-glucose/-galactose 4-epimerase under these growth conditions. In contrast, gal10(+) was efficiently expressed and involved in galactosylation of cell-surface proteins in low-glucose medium (0.1 % glucose and 2 % glycerol), but not in galactose-containing medium. In a uge1Deltagal10Delta strain, the galactosylation defect was suppressed and UDP-galactose content restored to wild-type levels in galactose-containing medium. Disruption of gal7(+), encoding galactose-1-phosphate uridylyltransferase, in the uge1Deltagal10Delta strain reversed suppression of the galactosylation defect and reduced levels of UDP-galactose, indicating that galactose is transported from the medium to the cytosol and is converted into UDP-galactose via galactose 1-phosphate by Gal7p in Sch. pombe. |
| 巻・号 | 156(Pt 3) |
| ページ | 708-718 |
| 公開日 | 2010-3-1 |
| DOI | 10.1099/mic.0.035279-0 |
| PMID | 19942659 |
| MeSH | Galactosephosphates / metabolism Gene Expression Regulation, Fungal Schizosaccharomyces / enzymology* Schizosaccharomyces / genetics Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* UDPglucose 4-Epimerase / genetics UDPglucose 4-Epimerase / metabolism* Uridine Diphosphate Galactose / metabolism |
| IF | 2.138 |
| 引用数 | 10 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 酵母 | S. pombe wild-type pREP1 pREP41? |