論文 - 詳細
| RRC ID | 12587 |
|---|---|
| 著者 | Ikeda Y, Ohashi T, Tanaka N, Takegawa K. |
| タイトル | Identification and characterization of a gene required for alpha1,2-mannose extension in the O-linked glycan synthesis pathway in Schizosaccharomyces pombe. |
| ジャーナル | FEMS Yeast Res |
| Abstract |
The KTRalpha1,2-mannosyltransferase gene family of Saccharomyces cerevisiae is responsible not only for outer-chain modifications of N-linked oligosaccharides but also for elongation of O-linked mannose residues. To identify genes involved in the elongation step of O-linked oligosaccharide chains in Schizosaccharomyces pombe, we characterized six genes, omh1(+)-omh6(+), that share significant sequence similarity to the S. cerevisiae KTR family. Six deletion strains were constructed, each carrying a single disrupted omh allele. All strains were viable, indicating that none of the omh genes was essential. Heterologous expression of a chitinase from S. cerevisiae in the omh mutants revealed that O-glycosylation of chitinase had decreased in omh1Delta cells, but not in the other mutants, indicating that the other omh genes do not appear to be required for O-glycan synthesis. Addition of the second alpha1,2-linked mannose residue was blocked in omh1Delta cells. An Omh1-GFP fusion protein was found to be localized in the Golgi apparatus. These results indicate that Omh1p plays a major role in extending alpha1,2-linked mannose in the O-glycan pathway in S. pombe. |
| 巻・号 | 9(1) |
| ページ | 115-25 |
| 公開日 | 2009-2-1 |
| DOI | 10.1111/j.1567-1364.2008.00458.x |
| PII | FYR458 |
| PMID | 19054127 |
| MeSH | Chitinases / metabolism Gene Deletion Genes, Reporter Glycosylation Golgi Apparatus / chemistry Green Fluorescent Proteins / analysis Green Fluorescent Proteins / genetics Mannose / metabolism* Mannosyltransferases / genetics* Mannosyltransferases / metabolism* Microbial Viability Microscopy, Fluorescence Polysaccharides / metabolism* Recombinant Fusion Proteins / analysis Recombinant Fusion Proteins / genetics Saccharomyces cerevisiae Proteins / genetics* Saccharomyces cerevisiae Proteins / metabolism* Schizosaccharomyces / enzymology* Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Sequence Homology, Amino Acid |
| IF | 3.193 |
| 引用数 | 19 |
| WOS 分野 | MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | plasmids? |