RRC ID 12589
Author Shioda N, Moriguchi S, Oya T, Ishii Y, Shen J, Matsushima T, Nishijo H, Sasahara M, Fukunaga K.
Title Aberrant hippocampal spine morphology and impaired memory formation in neuronal platelet-derived growth factor β-receptor lacking mice.
Journal Hippocampus
Abstract The physiological role of platelet-derived growth factor (PDGF) in the central nervous system (CNS) synaptic function remains uncharacterized. Here we identify physiological roles of PDGF receptor-β (PDGFR-β) in the CNS by conditional knockout of the gene encoding it. In the hippocampus, PDGFR-β colocalized immunohistochemically with both presynaptic synaptophysin and postsynaptic density-95 (PSD-95). In the hippocampal CA1 region, expression levels of postsynaptic proteins, including spinophilin, drebrin, and PSD-95, were significantly decreased in PDGFR-β knockout mice, although presynaptic synaptophysin levels remained comparable to controls. Interestingly, in hippocampal CA1 pyramidal neurons, dendritic spine density in PDGFR-β knockout mice was significantly decreased compared with that seen in wild-type mice, although spine length and number of dendritic branches remained unchanged. Consistent with these findings, impairment in hippocampal long-term potentiation (LTP) and in hippocampus-dependent memory formation were seen in PDGFR-β knockout mice. These results suggest PDGFR-β plays critical roles in spine morphology and memory formation in mouse brain.
Volume 22(6)
Pages 1371-8
Published 2012-6
DOI 10.1002/hipo.20973
PMID 21997856
MeSH Animals Dendritic Spines / metabolism* Dendritic Spines / pathology Hippocampus / metabolism* Hippocampus / pathology Memory Disorders / genetics Memory Disorders / metabolism* Memory Disorders / pathology Mice Mice, Knockout Neurons / metabolism* Neurons / pathology Random Allocation Receptor, Platelet-Derived Growth Factor beta / deficiency* Receptor, Platelet-Derived Growth Factor beta / genetics
IF 3.966
Times Cited 6
Mice C57BL/6-TgN(a-CaMKII-nlCre)/10