論文 - 詳細
| RRC ID | 12668 |
|---|---|
| 著者 | Mashimo T, Ohmori I, Ouchida M, Ohno Y, Tsurumi T, Miki T, Wakamori M, Ishihara S, Yoshida T, Takizawa A, Kato M, Hirabayashi M, Sasa M, Mori Y, Serikawa T. |
| タイトル | A missense mutation of the gene encoding voltage-dependent sodium channel (Nav1.1) confers susceptibility to febrile seizures in rats. |
| ジャーナル | J Neurosci |
| Abstract |
Although febrile seizures (FSs) are the most common convulsive syndrome in infants and childhood, the etiology of FSs has remained unclarified. Several missense mutations of the Na(v)1.1 channel (SCN1A), which alter channel properties, have been reported in a familial syndrome of GEFS+ (generalized epilepsy with febrile seizures plus). Here, we generated Scn1a-targeted rats carrying a missense mutation (N1417H) in the third pore region of the sodium channel by gene-driven ENU (N-ethyl-N-nitrosourea) mutagenesis. Despite their normal appearance under ordinary circumstances, Scn1a mutant rats exhibited remarkably high susceptibility to hyperthermia-induced seizures, which involve generalized clonic and/or tonic-clonic convulsions with paroxysmal epileptiform discharges. Whole-cell patch-clamp recordings from HEK cells expressing N1417H mutant channels and from hippocampal GABAergic interneurons of N1417H mutant rats revealed a significant shift of the inactivation curve in the hyperpolarizing direction. In addition, clamp recordings clearly showed the reduction in action potential amplitude in the hippocampal interneurons of these rats. These findings suggest that a missense mutation (N1417H) of the Na(v)1.1 channel confers susceptibility to FS and the impaired biophysical properties of inhibitory GABAergic neurons underlie one of the mechanisms of FS. |
| 巻・号 | 30(16) |
| ページ | 5744-53 |
| 公開日 | 2010-4-21 |
| DOI | 10.1523/JNEUROSCI.3360-09.2010 |
| PII | 30/16/5744 |
| PMID | 20410126 |
| PMC | PMC6632336 |
| MeSH | Amino Acid Sequence Animals Cell Line Genetic Predisposition to Disease / genetics* Humans Male Molecular Sequence Data Mutation, Missense / genetics* NAV1.1 Voltage-Gated Sodium Channel Nerve Tissue Proteins / genetics* Rats Rats, Inbred F344 Rats, Transgenic Seizures, Febrile / genetics* Seizures, Febrile / physiopathology Sodium Channels / genetics* |
| IF | 5.674 |
| 引用数 | 42 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ラット | F344-Scn1am1Kyo(strainID=716) |