RRC ID 12704
Author Yamada H, Ueda T, Yano A.
Title Water-soluble extract of Pacific Krill prevents triglyceride accumulation in adipocytes by suppressing PPARγ and C/EBPα expression.
Journal PLoS ONE
Abstract BACKGROUND:Pacific Krill (Euphausia pacifica) are small, red crustaceans, similar to shrimp, that flourish in the North Pacific and are eaten in Japan.
METHODS AND FINDINGS:We investigated the effect of a water-soluble extract of Pacific Krill on adipocytes and discovered that this extract suppressed triglyceride accumulation in adipocytes. Furthermore, the water-soluble extract of Pacific Krill suppressed the expression of two master regulators of adipocyte differentiation, peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT enhancer binding protein alpha (C/EBPα). C/EBPβ promotes PPARγ and C/EBPα expression, but the water-soluble extract of Pacific Krill did not inhibit the expression of C/EBPβ or C/EBPβ-mediated transcriptional activation. The Pacific Krill extract was more effective than a PPARγ antagonist in suppressing PPARγ and C/EBPα expression.
CONCLUSIONS:These results indicated that the water-soluble extract of Pacific Krill was not simply a PPARγ antagonist, but that it prevented triglyceride accumulation in adipocytes by suppression of PPARγ and C/EBPα via a pathway that is independent of C/EBPβ.
Volume 6(7)
Pages e21952
Published 2011
DOI 10.1371/journal.pone.0021952
PII PONE-D-11-00981
PMID 21760932
PMC PMC3131400
MeSH 3T3 Cells Adipocytes / cytology Adipocytes / drug effects Adipocytes / metabolism* Animals CCAAT-Enhancer-Binding Protein-alpha / genetics CCAAT-Enhancer-Binding Protein-alpha / metabolism* Cell Differentiation / drug effects Cell Proliferation / drug effects Euphausiacea / metabolism* Gene Expression Profiling Humans Mesenchymal Stromal Cells / cytology Mesenchymal Stromal Cells / drug effects Mesenchymal Stromal Cells / metabolism Mice PPAR gamma / antagonists & inhibitors PPAR gamma / genetics PPAR gamma / metabolism* Pacific Ocean RNA, Messenger / genetics RNA, Messenger / metabolism Solubility / drug effects Tissue Extracts / pharmacology* Transcriptional Activation / drug effects Triglycerides / metabolism* Water / chemistry*
IF 2.776
Times Cited 2
DNA material pC/EBP RE-TK hRluc(F) (RDB05595) pC/EBP RE-TK hRluc(R) (RDB05658)