論文 - 詳細
| RRC ID | 12704 |
|---|---|
| 著者 | Yamada H, Ueda T, Yano A. |
| タイトル | Water-soluble extract of Pacific Krill prevents triglyceride accumulation in adipocytes by suppressing PPARγ and C/EBPα expression. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:Pacific Krill (Euphausia pacifica) are small, red crustaceans, similar to shrimp, that flourish in the North Pacific and are eaten in Japan. METHODS AND FINDINGS:We investigated the effect of a water-soluble extract of Pacific Krill on adipocytes and discovered that this extract suppressed triglyceride accumulation in adipocytes. Furthermore, the water-soluble extract of Pacific Krill suppressed the expression of two master regulators of adipocyte differentiation, peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT enhancer binding protein alpha (C/EBPα). C/EBPβ promotes PPARγ and C/EBPα expression, but the water-soluble extract of Pacific Krill did not inhibit the expression of C/EBPβ or C/EBPβ-mediated transcriptional activation. The Pacific Krill extract was more effective than a PPARγ antagonist in suppressing PPARγ and C/EBPα expression. CONCLUSIONS:These results indicated that the water-soluble extract of Pacific Krill was not simply a PPARγ antagonist, but that it prevented triglyceride accumulation in adipocytes by suppression of PPARγ and C/EBPα via a pathway that is independent of C/EBPβ. |
| 巻・号 | 6(7) |
| ページ | e21952 |
| 公開日 | 2011-1-1 |
| DOI | 10.1371/journal.pone.0021952 |
| PII | PONE-D-11-00981 |
| PMID | 21760932 |
| PMC | PMC3131400 |
| MeSH | 3T3 Cells Adipocytes / cytology Adipocytes / drug effects Adipocytes / metabolism* Animals CCAAT-Enhancer-Binding Protein-alpha / genetics CCAAT-Enhancer-Binding Protein-alpha / metabolism* Cell Differentiation / drug effects Cell Proliferation / drug effects Euphausiacea / metabolism* Gene Expression Profiling Humans Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / drug effects Mesenchymal Stem Cells / metabolism Mice PPAR gamma / antagonists & inhibitors PPAR gamma / genetics PPAR gamma / metabolism* Pacific Ocean RNA, Messenger / genetics RNA, Messenger / metabolism Solubility / drug effects Tissue Extracts / pharmacology* Transcriptional Activation / drug effects Triglycerides / metabolism* Water / chemistry* |
| IF | 2.74 |
| 引用数 | 3 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 遺伝子材料 | pC/EBP RE-TK hRluc(F) (RDB05595) pC/EBP RE-TK hRluc(R) (RDB05658) |