RRC ID 12713
Author Nishimura I, Chano T, Kita H, Matsusue Y, Okabe H.
Title RB1CC1 protein suppresses type II collagen synthesis in chondrocytes and causes dwarfism.
Journal J Biol Chem
Abstract RB1-inducible coiled-coil 1 (RB1CC1) functions in various processes, such as cell growth, differentiation, senescence, apoptosis, and autophagy. The conditional transgenic mice with cartilage-specific RB1CC1 excess that were used in the present study were made for the first time by the Cre-loxP system. Cartilage-specific RB1CC1 excess caused dwarfism in mice without causing obvious abnormalities in endochondral ossification and subsequent skeletal development from embryo to adult. In vitro and in vivo analysis revealed that the dwarf phenotype in cartilaginous RB1CC1 excess was induced by reductions in the total amount of cartilage and the number of cartilaginous cells, following suppressions of type II collagen synthesis and Erk1/2 signals. In addition, we have demonstrated that two kinds of SNPs (T-547C and C-468T) in the human RB1CC1 promoter have significant influence on the self-transcriptional level. Accordingly, human genotypic variants of RB1CC1 that either stimulate or inhibit RB1CC1 transcription in vivo may cause body size variations.
Volume 286(51)
Pages 43925-43932
Published 2011-12-23
DOI 10.1074/jbc.M111.264192
PII S0021-9258(20)68485-X
PMID 22049074
PMC PMC3243532
MeSH Animals Autophagy-Related Proteins Body Size Cartilage / metabolism Chondrocytes / cytology* Collagen Type II / metabolism* Crosses, Genetic Dwarfism / genetics Gene Expression Regulation Humans Intracellular Signaling Peptides and Proteins / metabolism* Mice Mice, Inbred C57BL Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 / metabolism Protein-Tyrosine Kinases / metabolism*
IF 4.238
Times Cited 6
DNA material pCALNL5 (RDB01862)
Human and Animal Cells ATDC5(RCB0565)