Reference - Detail
| RRC ID | 1273 |
|---|---|
| Author | Li TC, Takeda N, Kato K, Nilsson J, Xing L, Haag L, Cheng RH, Miyamura T. |
| Title | Characterization of self-assembled virus-like particles of human polyomavirus BK generated by recombinant baculoviruses. |
| Journal | Virology |
| Abstract |
The major structural protein of the human polyomavirus BK (BKV), VP1, was expressed by using recombinant baculoviruses. A large amount of protein with a molecular mass of about 42 kDa was synthesized and identified by Western blotting. The protein was detected exclusively in the nuclei by immunofluorescent analysis and it was released into culture medium. The expressed BKV VP1 protein was self-assembled into virus-like particles (BK-VLPs) with two different sizes (50 and 26 nm in diameter), which migrated into four different bands in CsCl gradient with buoyant densities of 1.29, 1.30, 1.33, and 1.35 g/cm(3). The immunological studies on the BK-VLPs suggested that they have similar antigenicity with those of authentic BKV particles. Cryoelectron microscopy and 3D image analysis further revealed that the larger BK-VLPs were composed of 72 capsomers which all were pentamers arranged in a T = 7 surface lattice. This system provides useful information for detailed studies of viral morphogenesis and the structural basis for the antigenicity of BKV. |
| Volume | 311(1) |
| Pages | 115-24 |
| Published | 2003-6-20 |
| DOI | 10.1016/s0042-6822(03)00141-7 |
| PII | S0042682203001417 |
| PMID | 12832209 |
| MeSH | Amino Acid Sequence Animals BK Virus / chemistry BK Virus / physiology* BK Virus / ultrastructure Baculoviridae / genetics* Capsid / chemistry Capsid Proteins / biosynthesis Capsid Proteins / chemistry Capsid Proteins / genetics* Cell Line Cell Nucleus / metabolism Cloning, Molecular Cryoelectron Microscopy Culture Media Genetic Vectors* Humans Image Processing, Computer-Assisted Insecta Models, Molecular Molecular Sequence Data Molecular Weight Recombinant Proteins / biosynthesis Virus Assembly |
| IF | 2.819 |
| Times Cited | 41 |
| WOS Category | VIROLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | Sf9(RCB0563) |