Reference - Detail
| RRC ID | 12754 |
|---|---|
| Author | Saitoh Y, Martínez Bruyn VJ, Uota S, Hasegawa A, Yamamoto N, Imoto I, Inazawa J, Yamaoka S. |
| Title | Overexpression of NF-κB inducing kinase underlies constitutive NF-κB activation in lung cancer cells. |
| Journal | Lung Cancer |
| Abstract |
The present study investigates roles for NF-κB inducing kinase (NIK) in constitutive NF-κB activation in lung cancer cells. A wealth of evidence showed that NF-κB is often constitutively activated in human cancer cells, including non-small cell lung cancer tissue specimens and cell lines, which may lead to deregulated apoptosis and enhanced resistance of tumor cells to chemotherapy. However, the mechanisms of NF-κB activation in lung cancer cells remain largely unknown. We report here that NF-κB inducing kinase (NIK) is aberrantly expressed at the pre-translational level in non-small cell lung cancer (NSCLC) cell lines. Depletion of NIK by RNA interference remarkably diminished nuclear NF-κB DNA binding activity and reporter gene expression. NIK depletion induced apoptosis in A549 cells, reduced the matrix metalloproteinase 9 (MMP-9) and survivin mRNA expression and affected efficiency of anchorage-independent H1299 cell growth, suggesting a role for NIK in the manifestation of oncogenic phenotype. These results indicate that NIK plays a key role in constitutive NF-κB activation in NSCLC cells and implicate NIK as a molecular target for lung cancer therapy. |
| Volume | 70(3) |
| Pages | 263-70 |
| Published | 2010-12-1 |
| DOI | 10.1016/j.lungcan.2010.03.001 |
| PII | S0169-5002(10)00112-1 |
| PMID | 20338663 |
| MeSH | Apoptosis / genetics Carcinoma, Non-Small-Cell Lung / genetics* Carcinoma, Non-Small-Cell Lung / metabolism Carcinoma, Non-Small-Cell Lung / pathology Cell Growth Processes / genetics Cell Line, Tumor Gene Expression Regulation, Neoplastic Humans Inhibitor of Apoptosis Proteins / genetics Inhibitor of Apoptosis Proteins / metabolism Lung Neoplasms / genetics* Lung Neoplasms / metabolism Lung Neoplasms / pathology Matrix Metalloproteinase 9 / genetics Matrix Metalloproteinase 9 / metabolism NF-kappa B / genetics NF-kappa B / metabolism* Protein Binding / genetics Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* RNA, Small Interfering / genetics Survivin Transcriptional Activation* / genetics Transgenes / genetics |
| IF | 4.702 |
| Times Cited | 22 |
| WOS Category | RESPIRATORY SYSTEM ONCOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | CS-CDF-CG-PRE (RDB04379) |