論文 - 詳細
| RRC ID | 12755 |
|---|---|
| 著者 | Sumimoto H, Kawakami Y. |
| タイトル | The RNA silencing technology applied by lentiviral vectors in oncology. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Since the discovery of RNA interference (RNAi) in Caenorhabditis elegans in 1998, this mechanism has been found to be conserved in a wide variety of species, including insects, plants, and mammals. In mammals, small (or short) interfering RNA (siRNA) or short hairpin RNA (shRNA) can be expressed by using several expression vectors including lentiviral vectors. The lentiviral vector has several useful characteristics for RNAi experiments including broad host tropism and stable gene transduction to both dividing and nondividing cells, which permits stable depletion of target genes. This technology can be useful for several applications, including basic cancer research. |
| 巻・号 | 614 |
| ページ | 187-99 |
| 公開日 | 2010-1-1 |
| DOI | 10.1007/978-1-60761-533-0_13 |
| PMID | 20225045 |
| MeSH | Cell Line Gene Silencing* Genetic Vectors* Humans Lentivirus / genetics* Neoplasms / genetics Neoplasms / therapy RNA Interference RNA, Small Interfering / genetics* |
| 引用数 | 9 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) |