RRC ID 12756
Author Kiriyama M, Kobayashi Y, Saito M, Ishikawa F, Yonehara S.
Title Interaction of FLASH with arsenite resistance protein 2 is involved in cell cycle progression at S phase.
Journal Mol. Cell. Biol.
Abstract FLASH has been shown to be required for S phase progression and to interact with a nuclear protein, ataxia-telangiectasia locus (NPAT), a component of Cajal bodies in the nucleus and an activator of histone transcription. We investigated the role of human FLASH by using an inducible FLASH knockdown system in the presence or absence of various mutant forms of mouse FLASH. While carboxyl-terminal deletion mutants of FLASH, which do not interact with NPAT, can support S phase progression, its amino-terminal deletion mutants, which are unable to self associate, cannot support S phase progression, replication-dependent histone transcription, or the formation of Cajal bodies. Furthermore, FLASH was shown to be associated with arsenite resistance protein 2 (ARS2) through its central region, which is composed of only 13 amino acids. The expression of ARS2 and the interaction between FLASH and ARS2 are required for S phase progression. Taking these results together, FLASH functions in S phase progression through interaction with ARS2.
Volume 29(17)
Pages 4729-41
Published 2009-9
DOI 10.1128/MCB.00289-09
PII MCB.00289-09
PMID 19546234
PMC PMC2725714
MeSH Amino Acid Sequence Animals Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism* Cell Cycle / physiology* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Line Histones / genetics Histones / metabolism Humans Mice Molecular Sequence Data Nuclear Proteins / genetics Nuclear Proteins / metabolism* RNA / genetics RNA / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism S Phase / physiology* Sequence Alignment Transcription, Genetic
IF 3.735
Times Cited 31
DNA material CSII-EF-MCS (RDB04378)