RRC ID 12783
Author Li J, Zhang W, Wu H, Guo T, Liu X, Wan X, Jin J, Hanh TT, Thoa NT, Chen M, Liu S, Chen L, Liu X, Wang J, Zhai H, Wan J.
Title Fine mapping of stable QTLs related to eating quality in rice (Oryza sativa L.) by CSSLs harboring small target chromosomal segments.
Journal Breed. Sci.
Abstract Amylose content (AC) and viscosity profile are primary indices for evaluating eating and cooking qualities of rice grain. Using chromosome segment substitution lines (CSSLs), previous studies identified a QTL cluster of genes for rice eating and cooking quality in the interval R727-G1149 on chromosome 8. In this study we report two QTLs for viscosity parameters, respectively controlling setback viscosity (SBV) and consistency viscosity (CSV), located in the same interval using rapid viscosity analyzer (RVA) profile as an indicator of eating quality. Previously reported QTL for AC was dissected into two components with opposite genetic effects. Of four QTLs, qCSV-8 and qAC-8-2 had stable genetic effects across three and four environments, respectively. qSBV-8, qCSV-8 and qAC-8-1 partly overlapped, but were separated from qAC-8-2. Based on data from an Affymetrix rice GeneChip, two genes related to starch biosynthesis at the qAC-8-2 locus were chosen for further quantitative expression analysis. Both genes showed enhanced expression in sub-CSSLs carrying the target qAC-8-2 allele, but not in sub-CSSLs without the target qAC-8-2 allele, indicating their possible role in rice quality determination. Molecular markers closely linked to the two stable QTL provide the opportunity for marker-assisted selection (MAS) in breeding high quality rice.
Volume 61(4)
Pages 338-46
Published 2011-12
DOI 10.1270/jsbbs.61.338
PII bs-61-338
PMID 23136470
PMC PMC3406775
IF 1.559
Times Cited 8
WOS Category AGRONOMY PLANT SCIENCES
Resource
Rice CSSL