| RRC ID |
1291
|
| 著者 |
Okuhira K, Tsujita M, Yamauchi Y, Abe-Dohmae S, Kato K, Handa T, Yokoyama S.
|
| タイトル |
Potential involvement of dissociated apoA-I in the ABCA1-dependent cellular lipid release by HDL.
|
| ジャーナル |
J Lipid Res
|
| Abstract |
Helical apolipoproteins of high density lipoprotein (HDL) remove phospholipid and cholesterol from cells and generate HDL particles being mediated by ATP binding cassette transporter A1 (ABCA1). In murine macrophage cell line RAW264 cells, cAMP induced expression of ABCA1, release of cellular phospholipid and cholesterol by apolipoprotein A-I (apoA-I), and reversible binding of apoA-I to the cell. The apoA-I-dependent lipid release was directly proportional to the cAMP-induced binding of apoA-I, and was inhibited 70% by a monoclonal antibody selective to lipid-free apoA-I, 725-1E2. In contrast, apparent cellular cholesterol release to HDL was substantial even without ABCA1 induction, and it was increased only by 27% after the cAMP treatment. The antibody inhibited this increment by 70%. Lipid-free apoA-II liberated apoA-I from HDL by displacement and thereby markedly expanded the cAMP-induced part of the cholesterol release to the HDL-containing medium, and the antibody inhibited this part also by 70%. Binding experiments of the double-labeled reconstituted HDL showed that cAMP induced reversible binding of apoA-I but not the association of cholesteryl ester with the cells. The effect of the antibody on the cellular cholesterol release to the reconstituted HDL was similar to that of the HDL-mediated release. The data implicated that the ABCA1-dependent cholesterol release to HDL is mediated by apoA-I dissociated from HDL.
|
| 巻・号 |
45(4)
|
| ページ |
645-52
|
| 公開日 |
2004-4-1
|
| DOI |
10.1194/jlr.M300257-JLR200
|
| PII |
S0022-2275(20)31848-4
|
| PMID |
14729855
|
| MeSH |
ATP Binding Cassette Transporter 1
ATP-Binding Cassette Transporters / physiology*
Animals
Antibodies, Monoclonal / pharmacology
Apolipoprotein A-I / physiology*
Biological Transport
Cell Line
Cholesterol Esters / metabolism
Cyclic AMP / pharmacology
Lipid Metabolism*
Lipoproteins, HDL / metabolism*
Mice
Protein Binding / drug effects
|
| IF |
4.483
|
| 引用数 |
48
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
