| RRC ID |
1292
|
| 著者 |
Yamauchi Y, Chang CC, Hayashi M, Abe-Dohmae S, Reid PC, Chang TY, Yokoyama S.
|
| タイトル |
Intracellular cholesterol mobilization involved in the ABCA1/apolipoprotein-mediated assembly of high density lipoprotein in fibroblasts.
|
| ジャーナル |
J Lipid Res
|
| Abstract |
Differential regulation has been suggested for cellular cholesterol and phospholipid release mediated by apolipoprotein A-I (apoA-I)/ABCA1. We investigated various factors involved in cholesterol mobilization related to this pathway. ApoA-I induced a rapid decrease of the cellular cholesterol compartment that is in equilibrium with the ACAT-accessible pool in cells that generate cholesterol-rich HDL. Pharmacological and genetic inactivation of ACAT enhanced the apoA-I-mediated cholesterol release through upregulation of ABCA1 and through cholesterol enrichment in the HDL generated. Pharmacological activation of protein kinase C (PKC) also decreased the ACAT-accessible cholesterol pool, not only in the cells that produce cholesterol-rich HDL by apoA-I (i.e., human fibroblast WI-38 cells) but also in the cells that generate cholesterol-poor HDL (mouse fibroblast L929 cells). In L929 cells, the PKC activation caused an increase in apoA-I-mediated cholesterol release without detectable change in phospholipid release and in ABCA1 expression. These results indicate that apoA-I mobilizes intracellular cholesterol for the ABCA1-mediated release from the compartment that is under the control of ACAT. The cholesterol mobilization process is presumably related to PKC activation by apoA-I.
|
| 巻・号 |
45(10)
|
| ページ |
1943-51
|
| 公開日 |
2004-10-1
|
| DOI |
10.1194/jlr.M400264-JLR200
|
| PII |
S0022-2275(20)31268-2
|
| PMID |
15292375
|
| MeSH |
ATP Binding Cassette Transporter 1
ATP-Binding Cassette Transporters / metabolism*
Animals
Apolipoprotein A-I / metabolism*
Apolipoproteins / metabolism
Biological Transport
Cell Line
Cholesterol / metabolism*
Enzyme Activation
Fibroblasts / metabolism*
Humans
Lipoproteins, HDL / metabolism*
Protein Kinase C / metabolism
Sterol O-Acyltransferase / metabolism
Sterol O-Acyltransferase / physiology
|
| IF |
4.483
|
| 引用数 |
51
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
WI-38
COS-7(RCB0539) |
