| RRC ID |
1295
|
| Author |
Minekawa R, Takeda T, Sakata M, Hayashi M, Isobe A, Yamamoto T, Tasaka K, Murata Y.
|
| Title |
Human breast milk suppresses the transcriptional regulation of IL-1beta-induced NF-kappaB signaling in human intestinal cells.
|
| Journal |
Am J Physiol Cell Physiol
|
| Abstract |
Neonatal necrotizing enterocolitis (NEC), which is a disease with a poor prognosis, is considered to be caused by the coincidence of intestinal ischemia-reperfusion injury and systemic inflammation due to the colonization of pathogenic bacteria. Interleukin (IL)-8, a proinflammatory cytokine, plays an important role in the pathophysiology of NEC. It was recently reported that IL-1beta activates the IL-8 gene by regulating the transcriptional nuclear factor kappaB (NF-kappaB) signaling pathways in intestinal cells. The protective role of maternal milk in NEC pathogenesis has been reported in both human and animal studies. In this study, we show that human breast milk dramatically suppressed the IL-1beta-induced activation of the IL-8 gene promoter by inhibiting the activation pathway of NF-kappaB. Moreover, we also show that human breast milk induced the production of IkappaBalpha. These results suggest that human breast milk could be protective and therapeutic in neonates with NEC by inhibiting the activation pathway of NF-kappaB.
|
| Volume |
287(5)
|
| Pages |
C1404-11
|
| Published |
2004-11-1
|
| DOI |
10.1152/ajpcell.00471.2003
|
| PII |
00471.2003
|
| PMID |
15229109
|
| MeSH |
Blotting, Western
Caco-2 Cells
Electrophoresis, Polyacrylamide Gel
Enterocolitis, Necrotizing / physiopathology
Enzyme-Linked Immunosorbent Assay
Gene Expression / drug effects
Humans
Interleukin-1 / metabolism*
Interleukin-1 / pharmacology
Interleukin-8 / genetics
Interleukin-8 / metabolism
Intestinal Mucosa / metabolism
Milk, Human / metabolism*
NF-kappa B / drug effects
NF-kappa B / metabolism*
Signal Transduction / drug effects
Transcription, Genetic / drug effects
|
| IF |
3.485
|
| Times Cited |
32
|
|
WOS Category
|
PHYSIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
CACO-2(RCB0988) |
