| RRC ID |
1297
|
| Author |
Wada W, Maeshima A, Zhang YQ, Hasegawa Y, Kuwano H, Kojima I.
|
| Title |
Assessment of the function of the betaC-subunit of activin in cultured hepatocytes.
|
| Journal |
Am J Physiol Endocrinol Metab
|
| Abstract |
We assessed the function of the beta(C)-subunit of activin in hepatocytes. We studied the effect of conditioned medium of Chinese hamster ovary (CHO) cell line stably expressing the beta(C) gene (CHO-beta(C)) on growth of AML12 hepatocytes. We also examined the effect of recombinant activin C and transfection of the beta(C) gene by using adenovirus vector. CHO-beta(C) secreted activin C, a homodimer of the beta(C), as well as precursors of the beta(C). The conditioned medium of CHO-beta(C) increased both [(3)H]thymidine incorporation and the cell number in AML12 cells. It also supported survival of AML12 cells in a serum-free condition. Recombinant human activin C also increased both [(3)H]thymidine incorporation and the number of AML12 cells. Transfection of AML12 cells with the beta(C)-subunit led to the stimulation of [(3)H]thymidine incorporation. Analysis of the conditioned medium revealed that the beta(C)-subunit formed a heterodimer with the endogenous beta(A), the formation of which was dependent on the amount of beta(C) expressed. Recombinant activin C did not affect the binding of (125)I-activin A to its receptor or follistatin. These results indicate that activin C stimulates growth of AML12 cells. The beta(C)-subunit modifies the function of the beta(A)-subunit by multiple mechanisms.
|
| Volume |
287(2)
|
| Pages |
E247-54
|
| Published |
2004-8-1
|
| DOI |
10.1152/ajpendo.00390.2003
|
| PII |
00390.2003
|
| PMID |
15039147
|
| MeSH |
Animals
CHO Cells / metabolism
Cell Division / physiology
Cell Survival / physiology
Cells, Cultured
Cricetinae
Cricetulus
Culture Media, Conditioned / metabolism
Hepatocytes / cytology
Hepatocytes / metabolism*
Humans
Inhibin-beta Subunits / biosynthesis
Inhibin-beta Subunits / physiology*
Mice
Protein Conformation
Recombinant Proteins
Transfection
|
| IF |
3.469
|
| Times Cited |
21
|
|
WOS Category
|
PHYSIOLOGY
ENDOCRINOLOGY & METABOLISM
|
| Resource |
| Human and Animal Cells |
CHO-K1(RCB0285) |
