Reference - Detail
| RRC ID | 1297 |
|---|---|
| Author | Wada W, Maeshima A, Zhang YQ, Hasegawa Y, Kuwano H, Kojima I. |
| Title | Assessment of the function of the betaC-subunit of activin in cultured hepatocytes. |
| Journal | Am J Physiol Endocrinol Metab |
| Abstract |
We assessed the function of the beta(C)-subunit of activin in hepatocytes. We studied the effect of conditioned medium of Chinese hamster ovary (CHO) cell line stably expressing the beta(C) gene (CHO-beta(C)) on growth of AML12 hepatocytes. We also examined the effect of recombinant activin C and transfection of the beta(C) gene by using adenovirus vector. CHO-beta(C) secreted activin C, a homodimer of the beta(C), as well as precursors of the beta(C). The conditioned medium of CHO-beta(C) increased both [(3)H]thymidine incorporation and the cell number in AML12 cells. It also supported survival of AML12 cells in a serum-free condition. Recombinant human activin C also increased both [(3)H]thymidine incorporation and the number of AML12 cells. Transfection of AML12 cells with the beta(C)-subunit led to the stimulation of [(3)H]thymidine incorporation. Analysis of the conditioned medium revealed that the beta(C)-subunit formed a heterodimer with the endogenous beta(A), the formation of which was dependent on the amount of beta(C) expressed. Recombinant activin C did not affect the binding of (125)I-activin A to its receptor or follistatin. These results indicate that activin C stimulates growth of AML12 cells. The beta(C)-subunit modifies the function of the beta(A)-subunit by multiple mechanisms. |
| Volume | 287(2) |
| Pages | E247-54 |
| Published | 2004-8-1 |
| DOI | 10.1152/ajpendo.00390.2003 |
| PII | 00390.2003 |
| PMID | 15039147 |
| MeSH | Animals CHO Cells / metabolism Cell Division / physiology Cell Survival / physiology Cells, Cultured Cricetinae Cricetulus Culture Media, Conditioned / metabolism Hepatocytes / cytology Hepatocytes / metabolism* Humans Inhibin-beta Subunits / biosynthesis Inhibin-beta Subunits / physiology* Mice Protein Conformation Recombinant Proteins Transfection |
| IF | 3.469 |
| Times Cited | 21 |
| WOS Category | PHYSIOLOGY ENDOCRINOLOGY & METABOLISM |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | CHO-K1(RCB0285) |