RRC ID |
1299
|
著者 |
Mori K, Muto Y, Kokuzawa J, Yoshioka T, Yoshimura S, Iwama T, Okano Y, Sakai N.
|
タイトル |
Neuronal protein NP25 interacts with F-actin.
|
ジャーナル |
Neurosci Res
|
Abstract |
Neuronal protein NP25 is a neuron-specific protein present in highly differentiated neural cells, but its functional properties have not been well characterized. NP25 shows high amino acid sequence homology with the smooth muscle cell cytoskeleton-associated proteins, SM22, mp20, and calponin. To gain an insight into the biological functions of NP25, we first examined its subcellular localization in the human neuroblastoma cell line, SK-N-SH. NP25 diffusely distributed in the cytoplasm and fiber-like staining was also observed. It showed that NP25 co-localized with F-actin on stress fibers. A co-sedimentation assay demonstrated that NP25 bound to filamentous actin. Further investigations using fluorescence resonance energy transfer (FRET) technique revealed intracellular binding of NP25 and actin. The significance of the interaction between NP25 and F-actin is discussed.
|
巻・号 |
48(4)
|
ページ |
439-46
|
公開日 |
2004-4-1
|
DOI |
10.1016/j.neures.2003.12.012
|
PII |
S0168010203003481
|
PMID |
15041197
|
MeSH |
Actins / metabolism*
Animals
Blotting, Western
Cell Line, Tumor
Cytoskeleton / metabolism
Immunohistochemistry
Microscopy, Fluorescence
Nerve Tissue Proteins / metabolism*
Neuroblastoma / metabolism
|
IF |
2.645
|
引用数 |
24
|
WOS 分野
|
NEUROSCIENCES
|
リソース情報 |
ヒト・動物細胞 |
SK-N-SH(RCB0426) |