RRC ID |
12
|
著者 |
Jin G, Tsuruyama T, Yamada Y, Hiai H.
|
タイトル |
Svi3: A provirus common integration site in c-myc in SL/Kh pre-B lymphomas.
|
ジャーナル |
Cancer Sci
|
Abstract |
Spontaneous pre-B lymphomas in SL/Kh mice occur by somatic acquisition of a provirus genome of endogenous murine leukemia virus (MuLV). Inverse PCR amplification and sequence analyses of a provirus and its host flanking fragment revealed a proviral insertion into c-myc in 3 out of 60 SL/Kh pre-B lymphomas, named Svi3 lymphomas (SL/Kh virus integration site-3). Southern blot analysis revealed that two lymphomas had clonal integration in c-myc exon 1 and the other, in the promoter region. In 2 out of 3 Svi3 lymphomas, a fusion transcript of provirus 3' long terminal repeat and c-myc and a normal full-length c-myc transcript were obtained, but in one Svi3 lymphoma, only the normal transcript was obtained. All three Svi3 lymphomas had increased c-myc expression, producing normal 67-kDa c-Myc protein. Svi3 lymphomas had more mature phenotypes in the steps of early B-cell differentiation than Svi1 lymphomas, in which c-myc expression was indirectly up-regulated by provirus integration into Stat5a.
|
巻・号 |
94(9)
|
ページ |
791-5
|
公開日 |
2003-9-1
|
DOI |
10.1111/j.1349-7006.2003.tb01520.x
|
PMID |
12967477
|
MeSH |
3' Untranslated Regions / genetics
Animals
Blotting, Northern
Blotting, Southern
DNA Primers
DNA, Neoplasm / genetics
DNA-Binding Proteins / genetics*
Exons
Genes, myc / genetics*
Introns
Leukemia Virus, Murine / genetics*
Lymphoma, B-Cell / genetics*
Lymphoma, B-Cell / virology
Mice
Mice, Inbred Strains
Milk Proteins*
NIH 3T3 Cells
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
STAT5 Transcription Factor
Trans-Activators / genetics*
Transfection
Virus Integration*
|
IF |
4.966
|
引用数 |
8
|
WOS 分野
|
ONCOLOGY
|
リソース情報 |
実験動物マウス |
RBRC00222 |
ヒト・動物細胞 |
NIH3T3-3 |