論文 - 詳細
| RRC ID | 1310 |
|---|---|
| 著者 | Akasaki K, Nakamura N, Tsukui N, Yokota S, Murata S, Katoh R, Michihara A, Tsuji H, Marques ET Jr, August JT. |
| タイトル | Human dendritic cell lysosome-associated membrane protein expressed in lung type II pneumocytes. |
| ジャーナル | Arch Biochem Biophys |
| Abstract |
Human dendritic cell LAMP (hDC-LAMP) is a unique member of the lysosome-associated membrane protein (LAMP) family with a tissue distribution initially described as restricted to major histocompatibility class II (MHC II) compartments of activated DC before the translocation of MHC II to the cell surface [Immunity 9 (1998) 325]. In this report, we show that hDC-LAMP is also expressed by lung type II pneumocytes, another cell type with constitutive expression of MHC II. A recombinant hDC-LAMP protein and a monospecific anti-hDC-LAMP polyclonal antibody were prepared. The antibody reacted specifically with hDC-LAMP sequences of hDC-LAMP protein expressed in transfected cells and with a 54 kDa protein of normal human lung tissue with properties corresponding to those of transgene expressed hDC-LAMP. Immunohistochemical analysis of hDC-LAMP in human lung showed its presence in alveolar type II epithelial cells (type II pneumocytes) as well as in cells in the interfollicular area of bronchus-associated lymph nodes, where interdigitating DCs are concentrated, and with lesser staining of alveolar macrophages. The native protein contained approximately 16% carbohydrates, most of which are sialyl N-linked oligosaccharides, with an acidic isoelectric point (pI 4.8). The restricted localization of this protein to lung type II pneumocytes and DCs is in contrast to hLAMP-1, which was present in many cell types of the lung and lymph node. Type II pneumocytes are known to express MHC II and the abundant expression of hDC-LAMP in these cells as well as in DCs suggests its possible relationship to specific MHC II related function(s) of DC and type II pneumocytes. |
| 巻・号 | 425(2) |
| ページ | 147-57 |
| 公開日 | 2004-5-15 |
| DOI | 10.1016/j.abb.2004.02.042 |
| PII | S0003986104000797 |
| PMID | 15111122 |
| MeSH | Antigens, CD / chemistry* Antigens, CD / genetics Antigens, CD / metabolism* Cells, Cultured Dendritic Cells / metabolism* Histocompatibility Antigens Class II / chemistry Histocompatibility Antigens Class II / genetics Histocompatibility Antigens Class II / metabolism* Lung / metabolism* Lysosome-Associated Membrane Glycoproteins Molecular Weight Tissue Distribution Transfection / methods |
| IF | 3.391 |
| 引用数 | 11 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB00980 |