| RRC ID |
1310
|
| 著者 |
Akasaki K, Nakamura N, Tsukui N, Yokota S, Murata S, Katoh R, Michihara A, Tsuji H, Marques ET Jr, August JT.
|
| タイトル |
Human dendritic cell lysosome-associated membrane protein expressed in lung type II pneumocytes.
|
| ジャーナル |
Arch Biochem Biophys
|
| Abstract |
Human dendritic cell LAMP (hDC-LAMP) is a unique member of the lysosome-associated membrane protein (LAMP) family with a tissue distribution initially described as restricted to major histocompatibility class II (MHC II) compartments of activated DC before the translocation of MHC II to the cell surface [Immunity 9 (1998) 325]. In this report, we show that hDC-LAMP is also expressed by lung type II pneumocytes, another cell type with constitutive expression of MHC II. A recombinant hDC-LAMP protein and a monospecific anti-hDC-LAMP polyclonal antibody were prepared. The antibody reacted specifically with hDC-LAMP sequences of hDC-LAMP protein expressed in transfected cells and with a 54 kDa protein of normal human lung tissue with properties corresponding to those of transgene expressed hDC-LAMP. Immunohistochemical analysis of hDC-LAMP in human lung showed its presence in alveolar type II epithelial cells (type II pneumocytes) as well as in cells in the interfollicular area of bronchus-associated lymph nodes, where interdigitating DCs are concentrated, and with lesser staining of alveolar macrophages. The native protein contained approximately 16% carbohydrates, most of which are sialyl N-linked oligosaccharides, with an acidic isoelectric point (pI 4.8). The restricted localization of this protein to lung type II pneumocytes and DCs is in contrast to hLAMP-1, which was present in many cell types of the lung and lymph node. Type II pneumocytes are known to express MHC II and the abundant expression of hDC-LAMP in these cells as well as in DCs suggests its possible relationship to specific MHC II related function(s) of DC and type II pneumocytes.
|
| 巻・号 |
425(2)
|
| ページ |
147-57
|
| 公開日 |
2004-5-15
|
| DOI |
10.1016/j.abb.2004.02.042
|
| PII |
S0003986104000797
|
| PMID |
15111122
|
| MeSH |
Antigens, CD / chemistry*
Antigens, CD / genetics
Antigens, CD / metabolism*
Cells, Cultured
Dendritic Cells / metabolism*
Histocompatibility Antigens Class II / chemistry
Histocompatibility Antigens Class II / genetics
Histocompatibility Antigens Class II / metabolism*
Lung / metabolism*
Lysosome-Associated Membrane Glycoproteins
Molecular Weight
Tissue Distribution
Transfection / methods
|
| IF |
3.391
|
| 引用数 |
11
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
A549(RCB00980 |
