| RRC ID |
1316
|
| 著者 |
Ono K, Akatsu T, Murakami T, Wada S, Nishikawa M, Kugai N, Yamamoto M, Matsuura N, Nagata N.
|
| タイトル |
Mouse mammary carcinoma cell line (BALB/c-MC) stimulates osteoclast formation from mouse bone marrow cells through cell-to-cell contact.
|
| ジャーナル |
Bone
|
| Abstract |
We recently reported that numerous osteoclasts (OC) were formed in cocultures of some mouse cancer cell lines and bone marrow cells. In this study, we examined mechanisms by which one of the cell lines, BALB/c-MC, induces OC. BALB/c-MC dose dependently stimulated OC formation in cocultures. In cocultures where direct cell-to-cell contact between BALB/c-MC and bone marrow cells was inhibited by membrane filters, OC formation was not stimulated. The stimulation of OC formation in the coculture was completely abolished by adding 10(-7)-10(-6) mol/L indomethacin. The concentration of prostaglandin E2 (PGE2) in the culture media of cocultures with cell-to-cell contact was higher than that of cocultures without cell-to-cell contact or marrow cultures alone, and it reached levels sufficient to induce OC (11.9 +/- 5.3 ng/mL [about 3.4 x 10(-8) mol/L]). When BALB/c-MC or bone marrow cells were fixed with formalin and then cocultured with bone marrow cells or BALB/c-MC, respectively, the concentration of PGE2 in the culture media of cocultures of fixed BALB/c-MC and bone marrow cells increased, whereas that of cocultures of BALB/c-MC and fixed bone marrow cells did not increase. These results indicate that BALB/c-MC stimulate OC formation through direct cell-to-cell contact with bone marrow cells, and PGE2 released from bone marrow cells through direct cell-to-cell contact are involved in OC formation by the cell line.
|
| 巻・号 |
23(1)
|
| ページ |
27-32
|
| 公開日 |
1998-7-1
|
| DOI |
10.1016/s8756-3282(98)00065-9
|
| PII |
S8756-3282(98)00065-9
|
| PMID |
9662127
|
| MeSH |
Animals
Bone Marrow Cells / drug effects
Bone Marrow Cells / metabolism*
Cell Communication / physiology*
Coculture Techniques
Culture Media / chemistry
Dinoprostone / metabolism
Female
Indomethacin / pharmacology
Male
Mammary Neoplasms, Experimental / drug therapy
Mammary Neoplasms, Experimental / metabolism*
Mice
Mice, Inbred BALB C
Osteoclasts / physiology*
Tumor Cells, Cultured
|
| IF |
2.014
|
| 引用数 |
19
|
|
WOS 分野
|
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
mouse mammary carcinoma cell line (BALB/c-MC) |
