RRC ID 1320
Author Hashimoto K, Araki K, Osaki M, Nakamura H, Tomita K, Shimizu E, Ito H.
Title MCM2 and Ki-67 expression in human lung adenocarcinoma: prognostic implications.
Journal Pathobiology
Abstract OBJECTIVE:The expressions of minichromosome maintenance protein 2 (MCM2), Ki-67, and p53 were examined to analyze their pathobiological significance in human lung adenocarcinomas.
METHODS:We performed Western blot analysis in six human lung adenocarcinoma cell lines and immunohistochemistry in 145 surgically removed adenocarcinomas to examine the MCM2 expression. Labeling indices (LIs; %) of MCM2, Ki-67, and p53 in the tumor cells were compared with clinicopathological profiles and overall survival rates.
RESULTS:MCM2 protein was detected in all cell lines examined, with specific bands. MCM2 LIs were significantly correlated with sex, histological type, differentiation, pathological stage, and LIs of Ki-67 and p53 (p < 0.05). Significantly higher LIs of MCM2 and Ki-67 were noted in the 122 non-pure bronchioloalveolar carcinomas than in the 23 pure bronchioloalveolar carcinomas (p < 0.01), and the prognosis was poorer in the former than in the latter (p < 0.01). Sex, pathological stage, and high LIs of MCM2 and/or Ki-67 were independent prognostic factors (p < 0.05).
CONCLUSION:High LIs of MCM2 and/or Ki-67 suggest a poor prognosis in patients with lung adenocarcinoma (non-pure bronchioloalveolar carcinoma).
Volume 71(4)
Pages 193-200
Published 2004-1-1
DOI 10.1159/000078673
PII 78673
PMID 15263808
MeSH Adenocarcinoma, Bronchiolo-Alveolar / metabolism* Adenocarcinoma, Bronchiolo-Alveolar / mortality Adenocarcinoma, Bronchiolo-Alveolar / pathology Adult Aged Aged, 80 and over Biomarkers, Tumor / metabolism Blotting, Western Cell Cycle Proteins / metabolism* Cell Line, Tumor Female Humans Immunoenzyme Techniques Ki-67 Antigen / metabolism* Lung Neoplasms / metabolism* Lung Neoplasms / mortality Lung Neoplasms / pathology Male Middle Aged Minichromosome Maintenance Complex Component 2 Neoplasm Staging Nuclear Proteins / metabolism* Prognosis Survival Rate
IF 1.985
Times Cited 40
Human and Animal Cells A549(RCB0098) PC-14(RCB0446) RERF-LC-AI(RCB0444)