RRC ID 1330
著者 Terakawa M, Ogura M, Sato S, Wakisaka H, Ashida H, Uenoyama M, Masaki Y, Obara M.
タイトル Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave.
ジャーナル Opt Lett
Abstract Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (approximately 43 degrees C) was effective in improving the transfection efficiency.
巻・号 29(11)
ページ 1227-9
公開日 2004-6-1
DOI 10.1364/ol.29.001227
PMID 15209255
MeSH Animals Cell Membrane / metabolism* Cell Membrane / radiation effects* Cell Membrane Permeability / radiation effects* Gene Transfer Techniques Genetic Therapy / methods Green Fluorescent Proteins Lasers* Luminescent Proteins / genetics Luminescent Proteins / metabolism Mice NIH 3T3 Cells Plasmids / administration & dosage* Plasmids / genetics Plasmids / pharmacokinetics* Stress, Mechanical Temperature Transfection / methods*
IF 3.714
引用数 52
WOS 分野 OPTICS
リソース情報
ヒト・動物細胞 NIH3T3-3