論文 - 詳細
RRC ID | 1330 |
---|---|
著者 | Terakawa M, Ogura M, Sato S, Wakisaka H, Ashida H, Uenoyama M, Masaki Y, Obara M. |
タイトル | Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave. |
ジャーナル | Opt Lett |
Abstract |
Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (approximately 43 degrees C) was effective in improving the transfection efficiency. |
巻・号 | 29(11) |
ページ | 1227-9 |
公開日 | 2004-6-1 |
DOI | 10.1364/ol.29.001227 |
PMID | 15209255 |
MeSH | Animals Cell Membrane / metabolism* Cell Membrane / radiation effects* Cell Membrane Permeability / radiation effects* Gene Transfer Techniques Genetic Therapy / methods Green Fluorescent Proteins Lasers* Luminescent Proteins / genetics Luminescent Proteins / metabolism Mice NIH 3T3 Cells Plasmids / administration & dosage* Plasmids / genetics Plasmids / pharmacokinetics* Stress, Mechanical Temperature Transfection / methods* |
IF | 3.714 |
引用数 | 52 |
WOS 分野 | OPTICS |
リソース情報 | |
ヒト・動物細胞 | NIH3T3-3 |