RRC ID 1333
著者 Takase-Yoden S, Watanabe R.
タイトル Unique three-repeat sequences containing FVa, LVb/C4, and CORE motifs in LTR-U3 of Friend murine leukemia virus clone A8 accelerate the induction of thymoma in rat.
ジャーナル Virology
Abstract Friend murine leukemia virus (Fr-MLV) clone A8 causes thymoma 7 weeks postinfection in rats with a more rapid progression than clone 57. The U3 region of A8-LTR contains a unique structure of enhancer motifs consisting of three repeats of a 38-bp sequence containing FVa, LVb/C4, and CORE motifs. Replacement or deletion of the 38-bp sequence in the A8-U3 resulted in a marked reduction in tumorigenicity. Furthermore, the virus with 57-U3 gained high tumorigenicity after construction of the three 38-bp repeats in the U3 region. These findings indicated that the repeats of the 38-bp sequence of A8-LTR are essential for the rapid induction of thymoma. Interestingly, the repeat of the 38-bp sequence did not accelerate the amount of integrated viral DNA in the thymus during the early phase of infection, although it contributed to higher production of infectious virus. Thus, it was demonstrated that the ability to induce thymoma, which correlates with virus titer in the thymus, is not determined by the rate of viral DNA integration into the host genome.
巻・号 326(1)
ページ 29-40
公開日 2004-8-15
DOI 10.1016/j.virol.2004.04.047
PII S0042682204003526
PMID 15262492
MeSH Animals Animals, Newborn Base Sequence DNA, Viral / analysis Disease Models, Animal Enhancer Elements, Genetic Friend murine leukemia virus / genetics Friend murine leukemia virus / isolation & purification Friend murine leukemia virus / pathogenicity* Mice Molecular Sequence Data Mutation NIH 3T3 Cells Rats Rats, Inbred Lew Retroviridae Infections / virology* Sequence Alignment Terminal Repeat Sequences / genetics* Terminal Repeat Sequences / physiology Thymoma / virology* Thymus Neoplasms / virology* Tumor Virus Infections / virology* Virulence Virus Integration
IF 2.819
引用数 5
WOS 分野 VIROLOGY
リソース情報
ヒト・動物細胞 LYM-1