| RRC ID |
1354
|
| Author |
Nishimaki H, Kasai K, Kozaki Ki, Takeo T, Ikeda H, Saga S, Nitta M, Itoh G.
|
| Title |
A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Sonic hedgehog (Shh) is a secreted morphogen crucial for appropriate cellular proliferation during mammalian development. The activated Shh signaling is known to predispose to human tumors such as medulloblastoma and basal cell carcinoma, while a role of Shh signaling in the other common tumors is still controversial. Here we showed the overexpression of Shh in five cell lines among 14 human oral squamous cell carcinoma (OSCC) cell lines. One of the Shh-expressing OSCC cell lines HSQ-89 showed the inhibition of G1/S transition and apoptotic cell death by treatment with Cyclopamine, a steroidal alkaloid that blocks the intracellular Shh signaling. Furthermore, we found that treatment with Y-27632, a specific inhibitor of Rho-associated kinase, mimicked the effect of Cyclopamine on the cell cycle progression of HSQ-89. Our study revealed the involvement of activated Shh signaling in the cellular proliferation of OSCC cells, indicating Shh signaling might be a good therapeutic target for OSCC.
|
| Volume |
314(2)
|
| Pages |
313-20
|
| Published |
2004-2-6
|
| DOI |
10.1016/j.bbrc.2003.12.097
|
| PII |
S0006291X03027281
|
| PMID |
14733907
|
| MeSH |
Amides / pharmacology
Apoptosis
Blotting, Western
Carcinoma, Squamous Cell / metabolism
Cell Cycle
Cell Division
Cell Line, Tumor
Cell Separation
Culture Media, Conditioned / pharmacology
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Flow Cytometry
G1 Phase
Hedgehog Proteins
Humans
Intracellular Signaling Peptides and Proteins
Mouth Neoplasms / metabolism
Protein Serine-Threonine Kinases / metabolism
Pyridines / pharmacology
S Phase
Signal Transduction
Time Factors
Trans-Activators / metabolism*
Veratrum Alkaloids / pharmacology
rho-Associated Kinases
|
| IF |
2.985
|
| Times Cited |
52
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HSQ-89(RCB0789)
Sa3(RCB0980)
SCCKN(RCB0441)
SCCTF(RCB0442)
T3M-1 clone2(RCB1015)
T3M-1 cl-10(RCB1017) |
