RRC ID 1381
著者 Notoya M, Otsuka E, Yamaguchi A, Hagiwara H.
タイトル Runx-2 is not essential for the vitamin D-regulated expression of RANKL and osteoprotegerin in osteoblastic cells.
ジャーナル Biochem Biophys Res Commun
Abstract The differentiation and activity of osteoclasts are positively and negatively controlled by receptor activator of nuclear factor-kappaB ligand (RANKL), which is expressed on the surface of osteoblasts and stromal cells, and its decoy receptor osteoprotegerin (OPG), which is secreted by osteoblasts and stromal cells, respectively. The expression of the genes for RANKL and OPG is regulated by 1alpha,25-dihydroxyvitamin D(3) [1alpha,25(OH)(2)D(3)]. Runt-related gene-2 (Runx-2) is essential for osteoblast differentiation and there are several reports that Runx-2 is involved in osteoclast formation. Therefore, to clarify the role of Runx-2 in osteoclastogenesis, we designed a series of experiments using C2 cells and C6 cells, which are derived from calvariae of runx2-deficient mice. Treatment of C2 cells and C6 cells with 1alpha,25(OH)(2)D(3) for 2-4 days increased and decreased the levels of expression of the mRNAs for RANKL and OPG, respectively, and the effects were dose-dependent. However, by day 8, the level of RANKL mRNA had fallen and that of OPG mRNA had risen. Furthermore, C6 cells induced the differentiation of mouse spleen cells into tartrate-resistant acid phosphatase-positive (TRAP-positive) multinucleated cells (osteoclast-like cells) in the presence of 10(-7)M 1alpha,25(OH)(2)D(3). Such formation of osteoclast-like cells was inhibited by exogenous OPG in a dose-dependent manner. Thus, our findings indicate that Runx-2 is not essential for the expression of RANKL and OPG, and the formation of osteoclast-like cells.
巻・号 324(2)
ページ 655-60
公開日 2004-11-12
DOI 10.1016/j.bbrc.2004.09.101
PII S0006-291X(04)02166-7
PMID 15474477
MeSH 3T3 Cells Acid Phosphatase / metabolism Animals Carrier Proteins / metabolism* Cell Differentiation Cell Line Cells, Cultured Cholecalciferol / metabolism Core Binding Factor Alpha 1 Subunit Core Binding Factor alpha Subunits Dose-Response Relationship, Drug Glycoproteins / metabolism* Isoenzymes / metabolism Membrane Glycoproteins / metabolism* Mice Neoplasm Proteins / metabolism Neoplasm Proteins / physiology* Osteoblasts / metabolism* Osteoclasts / metabolism Osteoprotegerin RANK Ligand RNA, Messenger / metabolism Receptor Activator of Nuclear Factor-kappa B Receptors, Cytoplasmic and Nuclear / metabolism* Receptors, Tumor Necrosis Factor Reverse Transcriptase Polymerase Chain Reaction Spleen / cytology Stromal Cells / metabolism Tartrate-Resistant Acid Phosphatase Transcription Factors / metabolism Transcription Factors / physiology* Vitamin D / metabolism*
IF 2.985
引用数 19
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 ST2(RCB0224) C2C12(RCB0987) MC3T3-E1(RCB1126)