| RRC ID |
1384
|
| 著者 |
Miyazawa J, Mitoro A, Kawashiri S, Chada KK, Imai K.
|
| タイトル |
Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity.
|
| ジャーナル |
Cancer Res
|
| Abstract |
Carcinoma cells of epithelial origin are predisposed to acquire a fibroblastic feature during progression of neoplasm referred to as the epithelial-mesenchymal transition. HMGA2 is an architectural transcriptional factor that is expressed in the undifferentiated mesenchyme and initiates mesenchymal tumor formation. However, the biological consequence of the expression in the pathology of epithelial-type carcinomas is controversial. The present study was conducted to dissect the expression pattern in oral squamous cell carcinomas. HMGA2 was detected exclusively in carcinoma cell lines and tissues, but not in normal keratinocytes and gingival, by conventional reverse transcription-PCR. Quantitative real-time reverse transcription-PCR demonstrated 160-fold more HMGA2 expression in carcinoma tissues than in normal gingiva and 11-fold more HMGA2 expression in carcinoma cell lines than in normal keratinocytes. HMGA2 expression was observed by immunohistochemistry in 73.8% of 42 carcinomas and localized to the invasive front, where the cells exhibit the epithelial-mesenchymal transition. Fourteen patients who had been classified into a group without lymph node metastasis were positive for HMGA2 staining, and the disease recurred. Furthermore, carcinomas from all 23 patients who died of tumor recurrence stained for HMGA2, and HMGA2 staining was correlated to long-term survival of patients (P < 0.01). Multivariate risk factor analysis demonstrated that HMGA2 expression was an independent prognostic value for disease-specific overall survival (P < 0.01). These results suggest that HMGA2 contributes to the aggressiveness of carcinoma and that detection of HMGA2 expression is a useful predictive and prognostic tool in clinical management of oral carcinomas.
|
| 巻・号 |
64(6)
|
| ページ |
2024-9
|
| 公開日 |
2004-3-15
|
| DOI |
10.1158/0008-5472.can-03-1855
|
| PMID |
15026339
|
| MeSH |
Carcinoma, Squamous Cell / metabolism*
Carcinoma, Squamous Cell / pathology
Carcinoma, Squamous Cell / secondary
Epithelial Cells / metabolism
Epithelial Cells / pathology
Female
Gene Expression Regulation, Neoplastic
Gingiva / metabolism
Gingiva / pathology
HMGA2 Protein / metabolism*
Humans
Keratinocytes / metabolism
Keratinocytes / pathology
Lymphatic Metastasis / pathology
Male
Mesoderm / metabolism*
Mesoderm / pathology
Middle Aged
Mouth / metabolism
Mouth / pathology
Mouth Neoplasms / metabolism*
Mouth Neoplasms / pathology
Neoplasm Invasiveness / pathology
Neoplasm Recurrence, Local / pathology
Neoplasm Staging
Prognosis
Reverse Transcriptase Polymerase Chain Reaction
Survival Rate
|
| IF |
9.727
|
| 引用数 |
156
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
SCCKN(RCB0441) |
