RRC ID 1391
Author Ito A, Sasaguri Y, Kitada S, Kusaka Y, Kuwano K, Masutomi K, Mizuki E, Akao T, Ohba M.
Title A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells.
Journal J Biol Chem
Abstract Bacillus thuringiensis crystal proteins, well known to be toxic to certain insects but not pathogenic to mammals, are used as insecticidal proteins in agriculture and forest management. We here identified a crystal protein that is non-insecticidal and non-hemolytic but has strong cytocidal activity against various human cells with a markedly divergent target specificity, e.g. highly cytotoxic to HepG2 and Jurkat and less cytotoxic to the normal hepatocyte (HC) and HeLa. In slices of liver and colon cancer tissues, the toxin protein preferentially killed the cancer cells, leaving other cells unaffected. The cytocidal effect of the protein is non-apoptotic with swelling and fragmentation of the susceptible cells, although the apoptotic process does occur when the cell damage proceeded slowly. The amino acid sequence deduced from the nucleotide sequence of the cloned gene of the protein has little sequence homology with the insecticidal crystal proteins of B. thuringiensis. These observations raise the presence of a new group of the B. thuringiensis toxin and the possibility of new applications for the protein in the medical field.
Volume 279(20)
Pages 21282-6
Published 2004-5-14
DOI 10.1074/jbc.M401881200
PII S0021-9258(20)66990-3
PMID 15026424
MeSH Bacillus thuringiensis / pathogenicity Bacillus thuringiensis Toxins Bacterial Proteins / toxicity* Bacterial Toxins* Calcium / pharmacology Carcinoma, Hepatocellular Cell Line Cell Line, Tumor Cell Survival / drug effects* Endotoxins / toxicity* HeLa Cells Hemolysin Proteins Hepatocytes / drug effects Humans Liver Neoplasms Molecular Sequence Data T-Lymphocytes / drug effects*
IF 4.238
Times Cited 67
Human and Animal Cells HL60(RCB0041) Jurkat(RCB0806) Sawano(RCB1152) Hep G2(RCB1648) MOLT-4(RCB0206) A549(RCB0098) MRC-5(RCB0218) HeLa(RCB0007) CACO-2(RCB0988) TCS(RCB0638)