| RRC ID |
1393
|
| 著者 |
Sasanuma H, Tatsuno A, Tsuji K, Hidano S, Morita S, Kitamura T, Kubo M, Kitamura D, Goitsuka R.
|
| タイトル |
Transcriptional regulation of SLP-76 family hematopoietic cell adaptor MIST/Clnk by STAT5.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
SLP-76-related adaptor protein MIST (also called Clnk) is expressed in a variety of cytokine-dependent hematopoietic cell lines of myeloid and lymphoid origin as well as some cytokine-independent mast cell lines. To understand the molecular mechanisms underlying the MIST gene expression, we have characterized the 5'-flanking region of the mouse MIST gene. We have identified an enhancer region (-773 to -709), which is active in P815 mast cells expressing the endogenous MIST gene, but not in EL-4 T cells lacking MIST expression. Outside of this enhancer region, one STAT element present in the MIST promoter (-44 to -36) was found to bind STAT5A when IC-2 mast cells were stimulated with IL-3. Mutation of this STAT element did not affect basal MIST promoter activity in P815 mast cells, but was required for STAT5-mediated activation of the MIST promoter. Furthermore, endogenous MIST gene expression was induced in mast cells by a constitutively activated form of STAT5A, but not by an active mutant of c-Kit receptor. These findings suggest that STAT5 is involved in cytokine-mediated up-regulation of MIST gene expression, probably in collaboration with other lineage-specific transcription factors that promote basal MIST expression in mast cells.
|
| 巻・号 |
321(1)
|
| ページ |
145-53
|
| 公開日 |
2004-8-13
|
| DOI |
10.1016/j.bbrc.2004.06.126
|
| PII |
S0006-291X(04)01422-6
|
| PMID |
15358227
|
| MeSH |
Adaptor Proteins, Signal Transducing
Animals
Base Sequence
Carrier Proteins / genetics*
Cell Line
DNA-Binding Proteins / metabolism*
Mast Cells / physiology*
Mice
Milk Proteins / metabolism*
Molecular Sequence Data
Phosphoproteins / metabolism*
Polymerase Chain Reaction
Protein Folding
RNA, Messenger / genetics
Restriction Mapping
STAT5 Transcription Factor
T-Lymphocytes / physiology
Trans-Activators / metabolism*
Transcription, Genetic / genetics*
Transcriptional Activation
|
| IF |
2.985
|
| 引用数 |
2
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
IC-2(RCB0102) |
