論文 - 詳細
| RRC ID | 1398 |
|---|---|
| 著者 | Futaki S, Niwa M, Nakase I, Tadokoro A, Zhang Y, Nagaoka M, Wakako N, Sugiura Y. |
| タイトル | Arginine carrier peptide bearing Ni(II) chelator to promote cellular uptake of histidine-tagged proteins. |
| ジャーナル | Bioconjug Chem |
| Abstract |
Arginine-rich peptide-mediated protein delivery into living cells is a novel technology for controlling cell functions with therapeutic potential. In this report, a novel approach for the intracellular delivery of histidine-tagged proteins was introduced where a Ni(II) chelate of octaarginine peptide bearing nitrilotriacetic acid [R8-NTA-Ni(II)] was used as a membrane-permeable carrier molecule. Significant internalization of histidine-tagged enhanced green fluorescent protein (EGFP) into HeLa cells was observed by confocal microscopic observation in the presence of R8-NTA-Ni(II). Nuclear condensation characteristic in apoptotic cell death was also induced in the cells treated with a histidine-tagged apoptosis-inducing peptide [pro-apoptotic domain peptide (PAD)], indicating that the cargo molecules really went through the membrane to reach the cytosol. The apoptosis-inducing activity of the peptide thus delivered was compared with that of the PAD peptide covalently connected with the octaarginine peptide. |
| 巻・号 | 15(3) |
| ページ | 475-81 |
| 公開日 | 2004-1-1 |
| DOI | 10.1021/bc034181g |
| PMID | 15149174 |
| MeSH | Apoptosis / drug effects Arginine / chemistry* Cell Survival / drug effects Drug Carriers / chemical synthesis Drug Carriers / chemistry Drug Carriers / pharmacokinetics* Drug Design Green Fluorescent Proteins / chemistry Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism HeLa Cells Histidine / chemistry* Humans Nickel / chemistry* Nitrilotriacetic Acid / chemical synthesis Nitrilotriacetic Acid / chemistry Nitrilotriacetic Acid / pharmacokinetics Oligopeptides / chemistry* Peptides / chemical synthesis Peptides / chemistry Peptides / pharmacokinetics* |
| IF | 4.031 |
| 引用数 | 52 |
| WOS 分野 | CHEMISTRY, ORGANIC BIOCHEMICAL RESEARCH METHODS CHEMISTRY, MULTIDISCIPLINARY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |