RRC ID |
1398
|
著者 |
Futaki S, Niwa M, Nakase I, Tadokoro A, Zhang Y, Nagaoka M, Wakako N, Sugiura Y.
|
タイトル |
Arginine carrier peptide bearing Ni(II) chelator to promote cellular uptake of histidine-tagged proteins.
|
ジャーナル |
Bioconjug Chem
|
Abstract |
Arginine-rich peptide-mediated protein delivery into living cells is a novel technology for controlling cell functions with therapeutic potential. In this report, a novel approach for the intracellular delivery of histidine-tagged proteins was introduced where a Ni(II) chelate of octaarginine peptide bearing nitrilotriacetic acid [R8-NTA-Ni(II)] was used as a membrane-permeable carrier molecule. Significant internalization of histidine-tagged enhanced green fluorescent protein (EGFP) into HeLa cells was observed by confocal microscopic observation in the presence of R8-NTA-Ni(II). Nuclear condensation characteristic in apoptotic cell death was also induced in the cells treated with a histidine-tagged apoptosis-inducing peptide [pro-apoptotic domain peptide (PAD)], indicating that the cargo molecules really went through the membrane to reach the cytosol. The apoptosis-inducing activity of the peptide thus delivered was compared with that of the PAD peptide covalently connected with the octaarginine peptide.
|
巻・号 |
15(3)
|
ページ |
475-81
|
公開日 |
2004-1-1
|
DOI |
10.1021/bc034181g
|
PMID |
15149174
|
MeSH |
Apoptosis / drug effects
Arginine / chemistry*
Cell Survival / drug effects
Drug Carriers / chemical synthesis
Drug Carriers / chemistry
Drug Carriers / pharmacokinetics*
Drug Design
Green Fluorescent Proteins / chemistry
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
HeLa Cells
Histidine / chemistry*
Humans
Nickel / chemistry*
Nitrilotriacetic Acid / chemical synthesis
Nitrilotriacetic Acid / chemistry
Nitrilotriacetic Acid / pharmacokinetics
Oligopeptides / chemistry*
Peptides / chemical synthesis
Peptides / chemistry
Peptides / pharmacokinetics*
|
IF |
4.031
|
引用数 |
52
|
WOS 分野
|
CHEMISTRY, ORGANIC
BIOCHEMICAL RESEARCH METHODS
CHEMISTRY, MULTIDISCIPLINARY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
HeLa(RCB0007) |