論文 - 詳細
| RRC ID | 1414 |
|---|---|
| 著者 | Kasai A, Shintani N, Oda M, Kakuda M, Hashimoto H, Matsuda T, Hinuma S, Baba A. |
| タイトル | Apelin is a novel angiogenic factor in retinal endothelial cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
There has been much focus recently on the possible functions of apelin, an endogenous ligand for the orphan G-protein-coupled receptor APJ, in cardiovascular and central nervous systems. We report a new function of apelin as a novel angiogenic factor in retinal endothelial cells. The retinal endothelial cell line RF/6A highly expressed both apelin and APJ transcripts, while human umbilical venous endothelial cells (HUVECs) only expressed apelin mRNA. In accordance with these observations, apelin at concentrations of 1 pM-1 microM significantly enhanced migration, proliferation, and capillary-like tube formation of RF/6A cells, but not those of HUVECs, whereas VEGF stimulates those parameters of both cell types. In vivo Matrigel plug assay for angiogenesis, the inclusion of 1 nM apelin in the Matrigel resulted in clear capillary-like formations with an increase of hemoglobin content in the plug. This is the first report showing that apelin is an angiogenic factor in retinal endothelial cells. |
| 巻・号 | 325(2) |
| ページ | 395-400 |
| 公開日 | 2004-12-10 |
| DOI | 10.1016/j.bbrc.2004.10.042 |
| PII | S0006-291X(04)02324-1 |
| PMID | 15530405 |
| MeSH | Adipokines Angiogenesis Modulating Agents / metabolism Angiogenesis Modulating Agents / pharmacology Angiogenic Proteins / pharmacology* Angiogenic Proteins / physiology Animals Apelin Capillaries / growth & development Capillaries / ultrastructure Carrier Proteins / pharmacology* Carrier Proteins / physiology Cell Movement / drug effects Cell Proliferation / drug effects Endothelium, Vascular / cytology Endothelium, Vascular / drug effects Endothelium, Vascular / metabolism* Hemoglobins / analysis Humans Intercellular Signaling Peptides and Proteins Mice Neovascularization, Physiologic / drug effects Recombinant Proteins / genetics Recombinant Proteins / metabolism Recombinant Proteins / pharmacology Retina / cytology Retina / drug effects Retina / metabolism* Umbilical Veins / cytology Vascular Endothelial Growth Factor A / pharmacology |
| IF | 2.985 |
| 引用数 | 200 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RF/6A 135(RCB1556) |