| RRC ID |
1417
|
| Author |
Shou Z, Yamada K, Kawata H, Yokoyama O, Miyamoto K.
|
| Title |
A mechanism of induction of the mouse zinc-fingers and homeoboxes 1 (ZHX1) gene expression by interleukin-2.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The effect of IL-2 on the expression of the mouse zinc-fingers and homeoboxes 1 (ZHX1) gene was investigated in a mouse cytotoxic T cell line, CTLL-2 cells. IL-2 specifically induced the expression of ZHX1 mRNA. The level of ZHX1 mRNA was decreased in the absence of IL-2. These alterations were in parallel with the status of cell proliferation. The signaling pathways involved in the induction were examined. AG-490, wortmannin, and LY294002 blocked the induction by IL-2. Nuclear run-on assays and a mRNA stability analysis revealed that the half-life of ZHX1 mRNA but not the transcription rate of the gene was increased by IL-2. Thus, we conclude that IL-2 induces the expression of the mouse ZHX1 gene in CTLL-2 cells, that both Janus kinase 3/signal transducer and activator of transcription 5 and phosphoinositide 3-kinase pathways are involved in the induction, and that the increased mRNA stability results in the induction.
|
| Volume |
314(3)
|
| Pages |
885-90
|
| Published |
2004-2-13
|
| DOI |
10.1016/j.bbrc.2003.12.162
|
| PII |
S0006291X03027864
|
| PMID |
14741719
|
| MeSH |
Animals
Blotting, Northern
Cell Division / drug effects
Cell Line
Enzyme Inhibitors / pharmacology
Gene Expression Regulation / drug effects*
Homeodomain Proteins / biosynthesis*
Homeodomain Proteins / genetics*
Humans
Interleukin-2 / genetics
Interleukin-2 / pharmacology*
Mice
Protein-Tyrosine Kinases / antagonists & inhibitors
RNA, Messenger / analysis
RNA, Messenger / biosynthesis
Recombinant Proteins / pharmacology
Signal Transduction / drug effects
T-Lymphocytes, Cytotoxic / cytology
T-Lymphocytes, Cytotoxic / metabolism
Zinc Fingers / genetics*
|
| IF |
2.985
|
| Times Cited |
10
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
CTLL-2(RCB0637) |
