RRC ID 1425
著者 Ohuchida K, Mizumoto K, Ogura Y, Ishikawa N, Nagai E, Yamaguchi K, Tanaka M.
タイトル Quantitative assessment of telomerase activity and human telomerase reverse transcriptase messenger RNA levels in pancreatic juice samples for the diagnosis of pancreatic cancer.
ジャーナル Clin Cancer Res
Abstract Measurement of telomerase activity is a promising diagnostic tool for pancreatic cancer. Detection of mRNA for human telomerase reverse transcriptase (hTERT), a catalytic subunit of telomerase, is also a diagnostic candidate. In the present study, we developed a telomeric repeat amplification protocol assay with real-time PCR and a protocol for quantification of hTERT mRNA with real-time PCR. To evaluate the feasibility of these methods for diagnosis of pancreatic cancer, we measured telomerase activity and hTERT expression in pancreatic cancer cell lines, pancreatic tissues, and pancreatic juice samples from patients with different pancreatic diseases. There were significant correlations between telomerase activity and hTERT expression in cell lines, tissues, and juice samples. The levels of telomerase activity and hTERT expression were significantly higher in tumoral tissues than in nontumoral tissues. In pancreatic juice specimens, some carcinoma samples showed remarkably high expression of hTERT. However, there were no significant differences in hTERT expression between patients with carcinoma and those with benign diseases, although significant differences in telomerase activity were observed. Our present results suggest that the combined assessment of hTERT and telomerase activities in pancreatic juice provides a potent diagnostic method for pancreatic cancer.
巻・号 11(6)
ページ 2285-92
公開日 2005-3-15
DOI 10.1158/1078-0432.CCR-04-1581
PII 11/6/2285
PMID 15788678
MeSH Adenocarcinoma / genetics Adenocarcinoma / metabolism Adult Aged Aged, 80 and over Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism DNA-Binding Proteins Feasibility Studies Female Gene Expression Regulation, Enzymologic* Gene Expression Regulation, Neoplastic Humans Male Middle Aged Pancreatic Juice / enzymology* Pancreatic Neoplasms / genetics* Pancreatic Neoplasms / pathology Prognosis RNA / genetics* RNA / metabolism RNA, Messenger / metabolism* RNA, Neoplasm / metabolism* Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity Telomerase / genetics* Telomerase / metabolism Tumor Cells, Cultured
IF 10.107
引用数 44
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 MRC-5