| RRC ID |
1428
|
| Author |
Miyano J, Yamamoto S, Hanioka N, Narimatsu S, Ishikawa T, Ogura K, Watabe T, Nishimura M, Ueda N, Naito S.
|
| Title |
Involvement of SULT1A3 in elevated sulfation of 4-hydroxypropranolol in Hep G2 cells pretreated with beta-naphthoflavone.
|
| Journal |
Biochem Pharmacol
|
| Abstract |
Pretreatment of Hep G2 cells with beta-naphthoflavone (BNF 1-25microM) significantly increased cytosolic sulfation activities of 4-hydroxypropranolol (4-OH-PL) racemate. The profile was similar to those of sulfations towards dopamine and triiodothyronine in the same cytosolic fractions. Kinetic studies of 4-OH-PL sulfation in Hep G2 cytosolic fractions revealed that V(max) values increased but apparent K(m) values remained unchanged following the BNF pretreatment. Among five recombinant human SULT isoforms (SULT1A1, -1A3, -1B1, -1E1 and -2A1) examined, only SULT2A1 did not show 4-OH-PL sulfation activities under the conditions used. SULT1A3 and -1E1 exhibited an enantioselectivity of 4-OH-R-PL sulfation>4-OH-S-PL sulfation, which agreed with that of BNF-pretreated Hep G2 cells as well as of nontreated cells, whereas SULT1A1 and -1B1 showed a reversed enantioselectivity (R
|
| Volume |
69(6)
|
| Pages |
941-50
|
| Published |
2005-3-15
|
| DOI |
10.1016/j.bcp.2004.12.012
|
| PII |
S0006-2952(05)00004-3
|
| PMID |
15748705
|
| MeSH |
Arylsulfotransferase
Cell Line
Dose-Response Relationship, Drug
Humans
Isoenzymes / biosynthesis
Isoenzymes / genetics
Propranolol / analogs & derivatives*
Propranolol / metabolism*
Sulfates / metabolism
Sulfotransferases / biosynthesis*
Sulfotransferases / genetics
beta-Naphthoflavone / pharmacology*
|
| IF |
4.96
|
| Times Cited |
16
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
|
| Resource |
| Human and Animal Cells |
Hep G2 |
