RRC ID |
143
|
著者 |
Muramatsu Y, Yamada T, Taniguchi Y, Ogino T, Kose H, Matsumoto K, Sasaki Y.
|
タイトル |
Pnlip encoding pancreatic lipase is possible candidate for obesity QTL in the OLETF rat.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
The Otsuka Long-Evans Tokushima Fatty (OLETF) rat exhibits polygenic obesity, and one of quantitative trait loci (QTLs) responsible for a susceptibility to obesity in the OLETF, Nidd6/of, has been mapped to the approximately 10-cM genomic region between D1Rat166 and D1Rat90 on chromosome 1 in (OLETF x normal) F2 intercross. In this study, we have attempted to identify the causal gene for the Nidd6/of QTL. A Nidd6/of congenic strain, constructed by introgressing the OLETF allele on the mapped Nidd6/of region in the normal F344 rat strain, confirmed the existence of the Nidd6/of as obesity QTL. The Nidd6/of region was refined to a approximately 2.3-cM genomic region between D1Rat225 and D1Rat90, using informative recombinants selected from (Nidd6/of congenic x F344) F1 x Nidd6/of congenic backcross progenies. Among 46 genes located within the approximately 2.3-cM region, pancreatic lipase gene, Pnlip, was regarded as the most prominent and physiologically relevant positional candidate for the Nidd6/of QTL. We found that Pnlip possesses an OLETF allele-specific increase of mRNA levels in the pancreas, and that the OLETF allele is longer in variable number of tandem repeat (VNTR) within the 5'-flanking region than normal alleles. We further showed that the Nidd6/of QTL completely cosegregates with Pnlip VNTR in the informative recombinants from (Nidd6/of congenic x F344) F1 x Nidd6/of congenic backcross progenies. These results suggest that Pnlip is possible candidate for the Nidd6/of QTL.
|
巻・号 |
331(4)
|
ページ |
1270-6
|
公開日 |
2005-6-17
|
DOI |
10.1016/j.bbrc.2005.04.040
|
PII |
S0006-291X(05)00820-X
|
PMID |
15883013
|
MeSH |
Animals
Base Sequence
DNA Primers
Lipase / genetics*
Minisatellite Repeats
Obesity / enzymology
Obesity / genetics*
Pancreas / enzymology*
Polymorphism, Genetic
Quantitative Trait Loci*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rats
|
IF |
2.985
|
引用数 |
7
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ラット |
F344.OLETF-(D1Rat166-D1Rat90)/Tj(strainID=93) |