論文 - 詳細
| RRC ID | 143 |
|---|---|
| 著者 | Muramatsu Y, Yamada T, Taniguchi Y, Ogino T, Kose H, Matsumoto K, Sasaki Y. |
| タイトル | Pnlip encoding pancreatic lipase is possible candidate for obesity QTL in the OLETF rat. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The Otsuka Long-Evans Tokushima Fatty (OLETF) rat exhibits polygenic obesity, and one of quantitative trait loci (QTLs) responsible for a susceptibility to obesity in the OLETF, Nidd6/of, has been mapped to the approximately 10-cM genomic region between D1Rat166 and D1Rat90 on chromosome 1 in (OLETF x normal) F2 intercross. In this study, we have attempted to identify the causal gene for the Nidd6/of QTL. A Nidd6/of congenic strain, constructed by introgressing the OLETF allele on the mapped Nidd6/of region in the normal F344 rat strain, confirmed the existence of the Nidd6/of as obesity QTL. The Nidd6/of region was refined to a approximately 2.3-cM genomic region between D1Rat225 and D1Rat90, using informative recombinants selected from (Nidd6/of congenic x F344) F1 x Nidd6/of congenic backcross progenies. Among 46 genes located within the approximately 2.3-cM region, pancreatic lipase gene, Pnlip, was regarded as the most prominent and physiologically relevant positional candidate for the Nidd6/of QTL. We found that Pnlip possesses an OLETF allele-specific increase of mRNA levels in the pancreas, and that the OLETF allele is longer in variable number of tandem repeat (VNTR) within the 5'-flanking region than normal alleles. We further showed that the Nidd6/of QTL completely cosegregates with Pnlip VNTR in the informative recombinants from (Nidd6/of congenic x F344) F1 x Nidd6/of congenic backcross progenies. These results suggest that Pnlip is possible candidate for the Nidd6/of QTL. |
| 巻・号 | 331(4) |
| ページ | 1270-6 |
| 公開日 | 2005-6-17 |
| DOI | 10.1016/j.bbrc.2005.04.040 |
| PII | S0006-291X(05)00820-X |
| PMID | 15883013 |
| MeSH | Animals Base Sequence DNA Primers Lipase / genetics* Minisatellite Repeats Obesity / enzymology Obesity / genetics* Pancreas / enzymology* Polymorphism, Genetic Quantitative Trait Loci* RNA, Messenger / genetics RNA, Messenger / metabolism Rats |
| IF | 2.985 |
| 引用数 | 7 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ラット | F344.OLETF-(D1Rat166-D1Rat90)/Tj(strainID=93) |