論文 - 詳細
| RRC ID | 1436 |
|---|---|
| 著者 | Masuda S, Murakami M, Takanezawa Y, Aoki J, Arai H, Ishikawa Y, Ishii T, Arioka M, Kudo I. |
| タイトル | Neuronal expression and neuritogenic action of group X secreted phospholipase A2. |
| ジャーナル | J Biol Chem |
| Abstract |
Although individual mammalian secreted phospholipase A(2) (sPLA(2)) enzymes exhibit unique tissue and cellular distributions, the cell type-specific functions of each enzyme remain largely unknown. In this study, we found by immunohistochemistry that group X sPLA(2) (sPLA(2)-X) is uniquely located in the peripheral neuronal fibers, an observation that was supported by detection of its transcript and protein in the neuronal cell line PC12 and in primary dorsal root ganglia neurons. Adenoviral expression of sPLA(2)-X in PC12 cells facilitated neurite outgrowth, particularly when combined with a suboptimal concentration of nerve growth factor. In neuronally differentiated PC12 cells, sPLA(2)-X was preferentially localized in the Golgi apparatus and growth cones, and proteolytic conversion of the proenzyme to mature enzyme mainly occurred after the secretion process. The neurite-extending ability of sPLA(2)-X depended on the production of its catalytic product, lysophosphatidylcholine. Moreover, nerve growth factor-induced neurite extension of PC12 cells was modestly but significantly attenuated by an anti-sPLA(2)-X antibody or by a small interfering RNA for sPLA(2)-X. These observations suggest the potential contribution of sPLA(2)-X to neuronal differentiation, and possibly repair, under certain conditions. |
| 巻・号 | 280(24) |
| ページ | 23203-14 |
| 公開日 | 2005-6-17 |
| DOI | 10.1074/jbc.M500985200 |
| PII | S0021-9258(20)61495-8 |
| PMID | 15781456 |
| MeSH | Adenoviridae / metabolism Animals Blotting, Northern Catalysis Cell Differentiation Cell Line Chromatography, Thin Layer DNA, Complementary / metabolism Ganglia, Spinal / metabolism Golgi Apparatus / metabolism Humans Immunoblotting Immunohistochemistry Lysophosphatidylcholines / metabolism Mice Mice, Inbred C57BL Microscopy, Confocal Nerve Growth Factor Neurons / enzymology* Neurons / metabolism PC12 Cells Phospholipases A / biosynthesis* Phospholipases A / metabolism* Phospholipases A2 RNA, Small Interfering / metabolism Rats Reverse Transcriptase Polymerase Chain Reaction Spectrometry, Mass, Electrospray Ionization Time Factors |
| IF | 4.238 |
| 引用数 | 49 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |