論文 - 詳細
| RRC ID | 1439 |
|---|---|
| 著者 | Hassan F, Islam S, Mu MM, Ito H, Koide N, Mori I, Yoshida T, Yokochi T. |
| タイトル | Lipopolysaccharide prevents doxorubicin-induced apoptosis in RAW 264.7 macrophage cells by inhibiting p53 activation. |
| ジャーナル | Mol Cancer Res |
| Abstract |
The effect of lipopolysaccharide on doxorubicin-induced cell death was studied by using mouse RAW 264.7 macrophage cells. Pretreatment with lipopolysaccharide at 10 ng/mL prevented doxorubicin-induced cell death and the inhibition was roughly dependent on the concentration of lipopolysaccharide. Posttreatment with lipopolysaccharide for 1 hour also prevented doxorubicin-induced cell death. Lipopolysaccharide inhibited DNA fragmentation and caspase-3 activation in doxorubicin-treated RAW 264.7 cells, suggesting the prevention of doxorubicin-induced apoptosis. Lipopolysaccharide did not significantly inhibit doxorubicin-induced DNA damage detected by single-cell gel electrophoresis (comet) assay. Lipopolysaccharide definitely inhibited the stabilization and nuclear translocation of p53 in doxorubicin-treated RAW 264.7 cells. Lipopolysaccharide, as well as being an inhibitor of p53, abolished doxorubicin-induced apoptosis. Therefore, p53 was suggested to play a pivotal role in the prevention of doxorubicin-induced apoptosis in RAW 264.7 cells by lipopolysaccharide. |
| 巻・号 | 3(7) |
| ページ | 373-9 |
| 公開日 | 2005-7-1 |
| DOI | 10.1158/1541-7786.MCR-05-0046 |
| PII | 3/7/373 |
| PMID | 16046548 |
| MeSH | Animals Antibiotics, Antineoplastic / adverse effects Antibiotics, Antineoplastic / antagonists & inhibitors* Apoptosis / drug effects* Cell Line DNA Damage / drug effects DNA Fragmentation / drug effects Doxorubicin / adverse effects Doxorubicin / antagonists & inhibitors* Escherichia coli Humans Lipopolysaccharides / pharmacology* Macrophages / cytology Mice Mice, Inbred BALB C Tumor Suppressor Protein p53 / drug effects* Tumor Suppressor Protein p53 / physiology |
| IF | 4.63 |
| 引用数 | 28 |
| WOS 分野 | ONCOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |