RRC ID 1448
著者 Horie N, Hirabayashi N, Takahashi Y, Miyauchi Y, Taguchi H, Takeishi K.
タイトル Synergistic effect of green tea catechins on cell growth and apoptosis induction in gastric carcinoma cells.
ジャーナル Biol Pharm Bull
Abstract (-)-Epigallocatechin gallate (EGCG), a major component of green tea catechins, is known to inhibit cell growth and to induce apoptosis in a variety of cultured cells. We examined effects of green tea catechins in cultured cells derived from human gastric carcinoma. The proliferation of four cell lines (MKN-1, MKN-45, MKN-74 and KATO-III) was inhibited with EGCG in a dose-dependent manner. The growth of MKN-45 cells was most efficiently inhibited by the treatment (IC(50): 40 muM EGCG) among the four cell lines, while KATO-III cells were most insensitive (IC(50): 80-150 muM) to the EGCG treatment. In addition, (-)-epicatechin (EC) had a major synergistic effect on the induction of apoptosis in MKN-45 cells treated with EGCG; however it had little effect on the inhibition of cell growth induced by EGCG. To study the molecular mechanisms behind the induction of apoptosis by EGCG, the activity of caspases in MKN-45 cells treated with EGCG was examined. Activity levels of caspases-3, -8 and -9 were elevated in EGCG-treated cells, suggesting that these caspases are involved in the apoptosis induced by EGCG. Furthermore, the synergistic effect of EC with EGCG on the induction of apoptosis was specifically canceled by catalase treatment, suggesting that the synergism involves the extracellular production of reactive oxygen species.
巻・号 28(4)
ページ 574-9
公開日 2005-4-1
DOI 10.1248/bpb.28.574
PII JST.JSTAGE/bpb/28.574
PMID 15802789
MeSH Apoptosis / drug effects* Caspases / metabolism Catalase / metabolism Catechin / administration & dosage Catechin / analogs & derivatives* Catechin / pharmacology Cell Line, Tumor Cell Proliferation / drug effects* Dose-Response Relationship, Drug Enzyme Activation / drug effects Humans Stomach Neoplasms / drug therapy* Tea / chemistry*
IF 1.863
引用数 53
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 MKN1(RCB1003) MKN45(RCB1001) MKN74(RCB1002) Kato III(RCB2088) HeLa(RCB0007)