| RRC ID |
1450
|
| 著者 |
Koga T, Harada H, Shi TS, Okada S, Suico MA, Shuto T, Kai H.
|
| タイトル |
Hyperthermia suppresses the cytotoxicity of NK cells via down-regulation of perforin/granzyme B expression.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Hyperthermia, which is used as an adjunctive therapy for cancer, is known to modulate the activity of natural killer (NK) cells in vitro, but its effect in vivo is unclear. In the present study, we used a whole body hyperthermia (WBH) device heated by infrared rays to evaluate the effect of WBH on mice models. We demonstrate here that wild type C57BL/6J mice exposed to 42 degrees C for 60min had reduced NK cell cytolytic activity against YAC-1 target cells as determined by cytolytic assay. This result was confirmed using Rag-2 knockout mice, which possess functional NK but not cytolytic T or NK-T cells. Moreover, WBH decreased the mRNA expression of perforin and granzyme B in spleens of mice. But the expression of TNF cytokines (Fas ligand and TRAIL) was unchanged. These data suggest that the suppression of NK cell activity induced by WBH could be mediated through the perforin/granzyme pathway.
|
| 巻・号 |
337(4)
|
| ページ |
1319-23
|
| 公開日 |
2005-12-2
|
| DOI |
10.1016/j.bbrc.2005.09.184
|
| PII |
S0006-291X(05)02207-2
|
| PMID |
16236268
|
| MeSH |
Animals
Cell Line
Cell Proliferation
Cytotoxicity, Immunologic / immunology*
DNA-Binding Proteins / deficiency
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Down-Regulation*
Fever / physiopathology*
Granzymes
Killer Cells, Natural / immunology*
Killer Cells, Natural / metabolism*
Membrane Glycoproteins / genetics
Membrane Glycoproteins / metabolism*
Mice
Mice, Knockout
Perforin
Pore Forming Cytotoxic Proteins
Serine Endopeptidases / genetics
Serine Endopeptidases / metabolism*
Spleen / immunology
Spleen / metabolism
|
| IF |
2.985
|
| 引用数 |
16
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
YAC-1(RCB1165) |
