RRC ID 1469
著者 Sumita C, Yamane M, Matsuda T, Maeda M, Nariai T, Fujio Y, Azuma J.
タイトル Platelet activating factor induces cytoskeletal reorganization through Rho family pathway in THP-1 macrophages.
ジャーナル FEBS Lett
Abstract In the process of atherosclerosis, platelet activating factor (PAF) promotes the infiltration of inflammatory cells into atherosclerotic plaque by modulating their cytoskeleton. Here, we examined whether Rho family proteins are involved in PAF-induced cytoskeletal reorganization in THP-1 macrophages. PAF stimulation rapidly induced cell elongation, accompanied by filopodia formation. The inhibition of Rho family proteins by the overexpression of Rho-GDI attenuated the PAF-mediated morphological changes. Both RhoA and Cdc42 were activated in response to PAF. Inhibition of RhoA or Cdc42 by dominant negative mutants abrogated morphological changes induced by PAF. Collectively, PAF regulates cytoarchitecture through Rho family proteins in macrophages.
巻・号 579(18)
ページ 4038-42
公開日 2005-7-18
DOI 10.1016/j.febslet.2005.06.022
PII S0014-5793(05)00751-9
PMID 16004986
MeSH Adenoviridae / genetics Arteriosclerosis / metabolism Cell Line, Tumor Cytoskeleton / metabolism* Gene Transfer Techniques Guanine Nucleotide Dissociation Inhibitors / metabolism Guanosine Triphosphate / chemistry Guanosine Triphosphate / metabolism Humans Immunoblotting Inflammation Macrophages / metabolism* Platelet Activating Factor / metabolism* Pseudopodia / metabolism Time Factors Up-Regulation beta-Galactosidase / metabolism cdc42 GTP-Binding Protein / metabolism rac1 GTP-Binding Protein / metabolism rho GTP-Binding Proteins / metabolism* rho-Specific Guanine Nucleotide Dissociation Inhibitors rhoA GTP-Binding Protein / metabolism
IF 3.057
引用数 10
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 THP-1(RCB1189)