RRC ID 147
Author Sato Y, Ajiki T, Inoue S, Fujishiro J, Yoshino H, Igarashi Y, Hakamata Y, Kaneko T, Murakami T, Kobayashi E.
Title Gene silencing in rat-liver and limb grafts by rapid injection of small interference RNA.
Journal Transplantation
Abstract Genetic modification is a promising therapeutic strategy for organ transplantation. In previous studies, we introduced a nonviral method of gene transfer to donor grafts using an organ-selective injection technique to up-regulate gene expression. Based on the attractive methodology of RNA interference for silencing a particular gene expression, we applied our catheter-based injection method to transfer small interference RNA (siRNA)-green fluorescence protein (GFP) into liver and limb grafts. We first quantified the interfering activity after the systemic delivery of siRNA in the liver of Alb-DsRed2 transgenic (Tg) rats using in vivo bioimaging system. Then, using GFP Tg Lewis rats as donors, transient down-regulation of the GFP expression was achieved both in liver- and limb-transplantation models by the preoperative rapid injection of siRNA. Genetic modification by siRNA may provide new therapeutic options for down-regulation of endogenous antigenicity.
Volume 79(2)
Pages 240-3
Published 2005-1-27
PII 00007890-200501270-00017
PMID 15665774
MeSH Animals Animals, Genetically Modified Gene Silencing* Genes, Reporter Green Fluorescent Proteins / genetics Injections Liver Transplantation / physiology* RNA, Small Interfering / administration & dosage RNA, Small Interfering / genetics* Rats
IF 4.743
Times Cited 23